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XPC Antibody (3.26)

Images

 
Western Blot: XPC Antibody (3.26) [NB100-477] - DNA repair protein expression in tumors. A. Western blot determination of p53, XPC, XPA, ERCC1 and MSH2 in tumors. GAPDH is loading control. Image collected and cropped by ...read more
Western Blot: XPC Antibody (3.26) [NB100-477] - HeLa whole cell lysate detected using rabbit polyclonal .
Western Blot: XPC Antibody (3.26) [NB100-477] - Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: XPC Antibody (3.26) [NB100-477] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: XPC protein stained by XPC antibody [3.26] diluted at 1:100.Red: ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
3.26
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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XPC Antibody (3.26) Summary

Immunogen
Recombinant XPC purified from E. coli
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
XPC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000
Application Notes
IHC-P (Paraffin sections) Assay dependent
Theoretical MW
123 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Raji Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-477 in the following applications:

Publications
Read Publications using
NB100-477 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 17229834). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for XPC Antibody (3.26)

  • complementation group C antibody
  • p125
  • RAD4
  • Xeroderma pigmentosum group C-complementing protein
  • xeroderma pigmentosum, complementation group C
  • XP3
  • XPCCDNA repair protein complementing XP-C cells

Background

Human XPC (Xeroderma pigmentosum group C) is a member of a family of proteins that has been shown to be involved in the repair of DNA via the nucleotide excision repair (NER) pathway. Specifically, XPC is believed to be a part of a heteromeric protein complex that is involved in the recognition of the DNA lesions during global genomic repair but not transcription-coupled repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XPC Antibody (NB100-477)(21)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IF, WB, Western Blot.


Filter By Application
ICC/IF
(11)
IF
(1)
WB
(4)
Western Blot
(1)
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Filter By Species
Human
(16)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB100-477 Applications Species
Hameed J S F, Devarajan A, M S DP et al. PTEN-negative endometrial cancer cells protect their genome through enhanced DDB2 expression associated with augmented nucleotide excision repair BMC cancer 2023-05-04 [PMID: 37142958] (WB, Human) WB Human
Schaich MA, Schnable BL, Kumar N et al. Single-molecule analysis of DNA-binding proteins from nuclear extracts (SMADNE) Nucleic Acids Research 2023-04-24 [PMID: 36861323] (Western Blot) Western Blot
W Dai, A Wu, Y Li, G Yu, X Yan XPA Enhances Temozolomide Resistance of Glioblastoma Cells by Promoting Nucleotide Excision Repair Cell Transplantation, 2022-01-01;31(0):9636897221092. 2022-01-01 [PMID: 35536165]
W Sakai, M Yuasa-Suna, M Kusakabe, A Kishimoto, T Matsui, Y Kaneko, JI Akagi, N Huyghe, M Ikura, T Ikura, F Hanaoka, M Yokoi, K Sugasawa Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair Sci Rep, 2020-11-12;10(1):19704. 2020-11-12 [PMID: 33184426]
Fast OG, Gentry B, Strouth L et al. Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC Biosci. Rep. 2019-06-21 [PMID: 31227614] (IF, Human) IF Human
Beresova L, Vesela E, Chamrad I et al. Role of DNA Repair Factor Xeroderma Pigmentosum Protein Group C in Response to Replication Stress As Revealed by DNA Fragile Site Affinity Chromatography and Quantitative Proteomics. J. Proteome Res. 2016-12-02 [PMID: 27794614]
Mohni KN, Kavanaugh GM, Cortez D. ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency. Cancer Res. 2014-03-24 [PMID: 24662920] (WB, Human) WB Human
Maria Berra C, Santos de Oliveira C, Carriao Machado Garcia C et al. Nucleotide excision repair activity on DNA damage induced by photoactivated methylene blue. Free Radic Biol Med 2013-04-05 [PMID: 23567189] (WB, Human) WB Human
Kothandapani A, Gopalakrishnan K, Kahali B et al. Downregulation Of Swi/Snf Chromatin Remodeling Factor Subunits Modulate Cisplatin Cytotoxicity Exp Cell Res 2012-06-18 [PMID: 22721696] (ICC/IF, Human) ICC/IF Human
Muenyi CS, States VA, Masters JH et al. Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC). J Ovarian Res. 2011-06-01 [PMID: 21696631] (WB, Human) WB Human
Show All 21 Publications.

Review for XPC Antibody (NB100-477) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-477:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot XPC NB100-477
Enlarge
5
reviewed by:
Darshil Patel
WB Mouse 07/18/2017
View

Summary

ApplicationWestern Blot
Sample TestedMEFs whole cell lysate
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for XPC Antibody (NB100-477) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional XPC Products

Research Areas for XPC Antibody (NB100-477)

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Blogs on XPC

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Darshil Patel
07/18/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol XPC
Entrez
Uniprot