![Western Blot: XPF Antibody (4E11) [NBP2-01020] Analysis of extracts (35ug) from 9 different cell lines by using anti-XPF monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Western-Blot-NBP2-01020-img0016.jpg "Western Blot: XPF Antibody (4E11) [NBP2-01020] Analysis of extracts (35ug) from 9 different cell lines by using anti-XPF monoclonal antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI4E11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.84 mg/ml |
| Immunogen | Full length human recombinant protein of human ERCC4(NP_005227) produced in HEK293 cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ERCC4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 104.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.84 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: XPF Antibody (4E11) [NBP2-01020] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XPF.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunocytochemistry-Immunofluorescence-NBP2-01020-img0015.jpg "Immunocytochemistry/Immunofluorescence: XPF Antibody (4E11) [NBP2-01020] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XPF.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human tonsil using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0014.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human tonsil using anti-XPF mouse monoclonal antibody.")
![Flow Cytometry: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XPF antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Flow-Cytometry-NBP2-01020-img0001.jpg "Flow Cytometry: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XPF antibody, and then analyzed by flow cytometry.")
![Western Blot: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPF.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Western-Blot-NBP2-01020-img0002.jpg "Western Blot: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPF.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0003.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XPF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0004.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XPF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0005.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-XPF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0006.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XPF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0007.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-XPF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0008.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-XPF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0009.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-XPF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0010.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-XPF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human Kidney tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0011.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human Kidney tissue using anti-XPF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human lymphoma tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0012.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human lymphoma tissue using anti-XPF mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human prostate tissue using anti-XPF mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XPF-Antibody-4E11-Immunohistochemistry-Paraffin-NBP2-01020-img0013.jpg "Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human prostate tissue using anti-XPF mouse monoclonal antibody.")
