Home » CXCL2/GRO beta/MIP-2/CINC-3 » CXCL2 Antibodies » CXCL2/GRO beta/MIP-2/CINC-3 Antibody (123802)
Novus Biologicals products are now on bio-techne.com

CXCL2/GRO beta/MIP-2/CINC-3 Antibody (123802)

 (4 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Recombinant Rat CXCL2/CINC‑3 (Catalog # 525‑C3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/CINC‑3 (0.1 µg/mL) is neutral­ized (green line) by increasing concentrations of Mouse Anti-Rat CXCL2/CINC‑3 Monoclonal Antibody (Catalog # MAB525). The ND50 is typically 0.4‑2.0 µg/mL.
Recombinant Rat CXCL2/CINC‑3 (Catalog # 525‑C3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
123802
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB525-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CXCL2/GRO beta/MIP-2/CINC-3 Antibody (123802) Summary

Immunogen
E. coli-derived recombinant rat CXCL2/GRO beta /MIP‑2/CINC‑3
Ser32-Asn100
Accession # P30348
Specificity
Detects rat CXCL2/GRO beta /MIP‑2/CINC‑3 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant rat (rr) CXCL3 and less than 0.1% cross-reactivity with rrCXCL1, rrCINC-2 alpha , recombinant mouse (rm) CXCL9 or rmCXCL2 is observed. In Western blots, less than 3% cross-reactivity with recombinant human (rh) CXCL8, recombinant porcine CXCL8, or rrCINC-2 alpha and no cross‑reactivity with rrCXCL1, 3, rmCXCL1, 2, 6, 9, 10, 12, rhCXLC1, 2, 3, 5, 6, 7, 9,10, 11, 12 or 13 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CXCL2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.4-2.0 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Rat CXCL2/GRObeta /MIP-2/CINC-3 Biotinylated Antibody (Catalog number BAF525)
Standard: Recombinant Rat CXCL2/GRObeta /MIP-2/CINC-3 (Catalog number 525-C3)
Publications
Read Publications using
MAB525 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (123802)

  • chemokine (C-X-C motif) ligand 2
  • CINC-2a
  • CINC3
  • CINC-3
  • C-X-C Motif Chemokine 2
  • CXCL2
  • GRO beta
  • GRO2 oncogene
  • GRO2
  • GROB
  • Gro-beta
  • Growth-regulated protein beta
  • Macrophage inflammatory protein 2-alpha
  • melanoma growth stimulatory activity beta
  • MGSA beta
  • MGSA-b
  • MGSA-beta
  • MIP2
  • MIP-2
  • MIP2A
  • MIP-2a
  • MIP2-alpha
  • SCYB2

Background

Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.

CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.

  1. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
  2. Huang, S. et al. (1992) Am. J. Pathol. 141:981.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(811 Publications)
Species: Hu
Applications: BA
CXCL1/GRO
alpha/KC/CINC-1 [Biot
DY453
N/A CXCL1/GRO alpha/KC/CINC-1 [Biotin]
(290 Publications)
Species: Mu
Applications: ELISA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(661 Publications)
Species: Mu
Applications: ELISA
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(255 Publications)
Species: Hu
Applications: ELISA
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(183 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
Procalcitonin
Antibody (44D9)
NB120-14817
Procalcitonin Antibody (44D9)
(1 Review)
(11 Publications)
Species: Hu, Rt
Applications: ICC/IF, IHC, S-ELISA, WB

Myeloperoxidase/MPO An ...
AF3667
Western Blot Myeloperoxidase/MPO Antibody [Unconjugated]
Simple Western Myeloperoxidase/MPO Antibody [Unconjugated]
Immunocytochemistry Myeloperoxidase/MPO Antibody [Unconjugated]
(3 Reviews)
(167 Publications)
Species: Hu, Mu
Applications: Dual ISH-IHC, ICC, IHC, Simple Western, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(301 Publications)
Species: Mu
Applications: ELISA
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
CCL3/MIP-1 alpha
[HRP]
MMA00
N/A CCL3/MIP-1 alpha [HRP]
N/A CCL3/MIP-1 alpha [HRP]
(77 Publications)
Species: Mu
Applications: ELISA
NFkB p105/p50
Antibody (2J10D7) - B ...
NB100-56583
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
Flow Cytometry NFkB p105/p50 Antibody (2J10D7) - BSA Free
(1 Review)
(5 Publications)
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
CXCR2/IL-8RB
Antibody (48311) [Unc ...
MAB331
Immunohistochemistry CXCR2/IL-8RB Antibody (48311) [Unconjugated]
Flow Cytometry CXCR2/IL-8RB Antibody (48311) [Unconjugated]
Neutralization CXCR2/IL-8RB Antibody (48311) [Unconjugated]
(2 Reviews)
(66 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
Transportin 1
Antibody (D45) - BSA
NB600-1397
Western Blot Transportin 1 Antibody (D45) - BSA Free
Western Blot Transportin 1 Antibody (D45) - BSA Free
Immunohistochemistry-Paraffin Transportin 1 Antibody (D45) - BSA Free
(1 Review)
(7 Publications)
Species: Bv, Ca, Hu, Pm, Mu, Rb
Applications: CyTOF-ready, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, MiAr, WB
CXCL3/GRO
gamma/CINC-2/DCIP-1 [ ...
8297-GG/CF
Bioactivity CXCL3/GRO gamma/CINC-2/DCIP-1 [Unconjugated]
Species: Hu
Applications: BA
TLR4 Antibody
(76B357.1) - BSA Free
NB100-56566
Dual RNAscope ISH-IHC TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
Immunohistochemistry TLR4 Antibody (76B357.1) - BSA Free
(5 Reviews)
(140 Publications)
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
MIPEP Antibody
(OTI5E7)
NBP2-45861
Flow Cytometry MIPEP Antibody (OTI5E7)
Immunofluorescence MIPEP Antibody (OTI5E7)
Immunohistochemistry MIPEP Antibody (OTI5E7)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Aquaporin-0
Antibody - BSA Free
NBP2-92773
Immunohistochemistry-Paraffin Aquaporin-0 Antibody - BSA Free
Immunohistochemistry-Paraffin Aquaporin-0 Antibody - BSA Free
Western Blot Aquaporin-0 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
CXCL10/IP-10/CRG-2
[HRP]
DIP100
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
(155 Publications)
Species: Hu
Applications: ELISA

Publications for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (MAB525)(4)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ELISA Development.


Filter By Application
ELISA Development
(2)
All Applications
Filter By Species
Rat
(2)
All Species

Reviews for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (MAB525) (0)

There are no reviews for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (MAB525). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CXCL2/GRO beta/MIP-2/CINC-3 Antibody (MAB525) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CXCL2/GRO beta/MIP-2/CINC-3 Products

Blogs on CXCL2/GRO beta/MIP-2/CINC-3

There are no specific blogs for CXCL2/GRO beta/MIP-2/CINC-3, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CXCL2/GRO beta/MIP-2/CINC-3 Antibody (123802) and receive a gift card or discount.


Bioinformatics

Gene Symbol CXCL2
Uniprot