One of our customers wants to buy PCT antibodies for ELISA detection from Novus and he has searched all the PCT antibodies on Novus website and has some questions as follows: 1. NB110-16925 (On the Novus website, NBP1-79065 is recommended for coating and NB110-16925 is for Conjugate. I want to know whether you have done some validation about your suggestion to confirm this recommended pair for sandwich immunoassay is the best choice. If so, I will not try other optimization such as coating plate with NB110-16925 and using NBP1-79065 for conjugate so as not to waste time.) 2. NB120-14817 (My question is the same as NB110-16925 and items below is the recommendation from Novus website: Recommended pairs for sandwich immunoassay (coating - conjugate): NBP1-79064(Coating) - NB120-14817(Conjugate)). 3. NB110-13044 (Do you have any recommendation pairs for this antibody? There is no suggestion about sandwich immunoassay on Novus website.)

Name: Procalcitonin Antibody (44D9)
Brand: Novus Biologicals
SKU: NB120-14817
Rating: 5 (1 reviews)

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Accepted Answer

To answer your questions the antibodies that are recommended as a pair are in fact ones that have been tested together and shown as effective sandwich pairs. We will of course always guarantee all stated species and applications, but the ones that have indications to be used together are the ones we recommend for the customers. In regards to the last antibody I am not sure what pair was used for that because I do not see it in the notes so I cannot say with 100% certainty which one would be best to use for that one and would try to stick to the others.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, RtSpecies Glossary
Applications	WB, ELISA, ICC/IF, IHC
Clone	44D9
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB120-14817AF350

Alexa Fluor 405 NB120-14817AF405

Alexa Fluor 488 NB120-14817AF488

Alexa Fluor 532 NB120-14817AF532

Alexa Fluor 594 NB120-14817AF594

Alexa Fluor 647 NB120-14817AF647

Alexa Fluor 700 NB120-14817AF700

Alexa Fluor 750 NB120-14817AF750

Biotin NB120-14817B

DyLight 350 NB120-14817UV-200ul

DyLight 405 NB120-14817V

DyLight 488 NB120-14817G

DyLight 550 NB120-14817R

DyLight 594 NB120-14817DL594

DyLight 650 NB120-14817C

DyLight 680 NB120-14817FR

DyLight 755 NB120-14817IR

FITC NB120-14817F

HRP NB120-14817H

PerCP NB120-14817PCP

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB120-14817AF350
Alexa Fluor 405	NB120-14817AF405
Alexa Fluor 488	NB120-14817AF488
Alexa Fluor 532	NB120-14817AF532
Alexa Fluor 594	NB120-14817AF594
Alexa Fluor 647	NB120-14817AF647
Alexa Fluor 700	NB120-14817AF700
Alexa Fluor 750	NB120-14817AF750
Biotin	NB120-14817B
DyLight 350	NB120-14817UV-200ul
DyLight 405	NB120-14817V
DyLight 488	NB120-14817G
DyLight 550	NB120-14817R
DyLight 594	NB120-14817DL594
DyLight 650	NB120-14817C
DyLight 680	NB120-14817FR
DyLight 755	NB120-14817IR
FITC	NB120-14817F
HRP	NB120-14817H
PerCP	NB120-14817PCP

Immunogen	Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragments of human Procalcitonin conjugated with carrier protein.
Localization	Secreted
Isotype	IgG2a
Clonality	Monoclonal
Host	Mouse
Gene	CALCA
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Procalcitonin Antibody (44D9)

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Publications for Procalcitonin Antibody (NB120-14817)(11)

Review for Procalcitonin Antibody (NB120-14817) (1) 51

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Storage	Store at 4C. Do not freeze.
Buffer	PBS (pH 7.4)
Preservative	0.09% Sodium Azide
Purity	Protein A purified

Publications using NB120-14817	Applications	Species
Tavares E, Maldonado R, Garcia-Martinez A, Minano FJ. Central Administration of Aminoprocalcitonin Inhibits Food Intake and Stimulates the Hypothalamic-Pituitary-Adrenal Axis in Rats via the Corticotrophin-Releasing Factor System J Neuroendocrinol 2012-07-01 [PMID: 22372932] (WB, IF/IHC, ICC/IF, Rat)	WB, IF/IHC, ICC/IF	Rat
Selimo?lu F, Ayhan M Silver nanoparticle decorated graphene-based SERS electrode towards procalcitonin detection Vibrational Spectroscopy 2023-05-01
Song C, Zhao J, Hao J et al. Aminoprocalcitonin protects against hippocampal neuronal death via preserving oxidative phosphorylation in refractory status epilepticus Cell death discovery 2023-05-04 [PMID: 37142587] (WB, IHC-Fr, EM, Rat) Details: dilutions: EM: 1:100, IHC-Fr 1:100; WB 1:500	WB, IHC-Fr, EM	Rat
Zeng JT, Wang Y, Wang Y et al. Elevated procalcitonin levels in the absence of infection in procalcitonin-secretin hepatocellular carcinoma: A case report World Journal of Clinical Cases 2022-10-16 [PMID: 36312465] (IHC)	IHC
Ang SX, Chen CP, Sun FJ, Chen CY Bio-Functionalized Magnetic Nanoparticles for the Immunoassay of C-Reactive Protein and Procalcitonin in Cervicovaginal Secretions of Pregnant Women with Preterm Prelabor Rupture of Membranes to Predict Early-Onset Neonatal Sepsis International journal of nanomedicine 2022-01-20 [PMID: 35082495]
Ma S, Wang H, Li W Et al. The correlation between the expression of ATF4 and procalcitonin combined with the detection of RET mutation and the pathological stage and clinical prognosis of medullary thyroid carcinoma Canadian journal of physiology and pharmacology 2021-11-25 [PMID: 34822305]
Wang W, Xu L, Hu D, Tang W Case of an abnormal procalcitonin producing metastatic pancreatic neuroendocrine tumor Clin Case Rep 2020-11-25 [PMID: 33235774]
Hui-Heng L, Jun-Sheng W, Jin-Zhou L et al. Lupeol Counteracts the Proinflammatory Signalling Triggered in Macrophages by 7-Keto-Cholesterol: New Perspectives in the Therapy of Atherosclerosis Cytokine 2020-09-27 [PMID: 33039977] (WB, Rat)	WB	Rat
Takahashi K, Ozawa E et al. Hepatobiliary and Pancreatic: A procalcitonin-secreting and calcitonin-secreting pancreatic neuroendocrine carcinoma. J Gastroenterol Hepatol 2019-01-06 [PMID: 30663800] (IF/IHC, Human)	IF/IHC	Human
Luohai Chen, Yu Zhang, Yuan Lin et al. The role of elevated serum procalcitonin in neuroendocrine neoplasms of digestive system. Clinical Biochemistry 2017-06-26 [PMID: 28668469] (IF/IHC, Human)	IF/IHC	Human
Tavares E, Maldonado R, Minano FJ. Immunoneutralization of Endogenous Aminoprocalcitonin Attenuates Sepsis-Induced Acute Lung Injury and Mortality in Rats. Am. J. Pathol. 2014-09-20 [PMID: 25251058] Details: Procalcitonin/ aminoprocalcitonin or NPCT antibody used for WB at 1:2000 dilution on Rat's lung tissues - Figure 1B shows a specific 8kDa band representing Rat's NPCT
Show All 11 Publications. Collapse Publications.

Gene Symbol	CALCA
Entrez	796(Human)
Uniprot	P06881(Human)

	Verified Customer 07/15/2017
Application:	IHC-P
Species:	Human