Home » CXCR2/IL-8RB » CXCR2/IL-8RB Antibodies » CXCR2/IL-8RB Antibody (48311) [Unconjugated]
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CXCR2/IL-8RB Antibody (48311) [Unconjugated]

 (66 Publications)
 (2 Reviews)
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Human peripheral blood granulocytes were stained with Mouse Anti-HumanCXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Human peripheral blood granulocytes were stained with Mouse Anti-HumanCXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), ...read more
CXCR2/IL-8 RB was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331) overnight at 4 °C. Tissue was stained ...read more
Recombinant Human CXCL1/GRO alpha (Catalog #275-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready
Clone
48311
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB331U-100UG
Alexa Fluor 405
FAB331V-100UG
Alexa Fluor 488
FAB331G-100UG
Alexa Fluor 532
FAB331X
Alexa Fluor 594
FAB331T-100UG
Alexa Fluor 647
FAB331R-100UG
Alexa Fluor 700
FAB331N-100UG
Alexa Fluor 750
FAB331S-100UG
Allophycocyanin
FAB331A-025
Allophycocyanin
FAB331A-100
Allophycocyanin/Cy7
FAB331APCCY7
Biotin
FAB331B
CoraFluor 1
FAB331CL1
DyLight 350
FAB331D
DyLight 405
FAB331E
DyLight 488
FAB331K
DyLight 550
FAB331L
DyLight 594
FAB331M
DyLight 650
FAB331W
DyLight 680
FAB331Y
DyLight 755
FAB331Z
Fluorescein
FAB331F-100
Fluorescein
FAB331F-025
HRP
FAB331H
Janelia Fluor 525
FAB331JF525
Janelia Fluor 549
FAB331I
Janelia Fluor 646
FAB331J
Janelia Fluor 669
FAB331JF669
PE/Atto594
FAB331PEATT594
PE/Cy5.5
FAB331PECY55
PE/Cy7
FAB331PECY7
Phycoerythrin
FAB331P-100
Phycoerythrin
FAB331P-025
mFluor Violet 450 SE
FAB331MFV450
mFluor Violet 500 SE
FAB331MFV500
mFluor Violet 610 SE
FAB331MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB331V-100UG
Carrier Free
MAB331-500
Carrier Free
FAB331G-100UG
Carrier Free
FAB331R-100UG
Carrier Free
FAB331S-100UG
Carrier Free
FAB331T-100UG
Carrier Free
FAB331U-100UG
Carrier Free
MAB331-SP
Carrier Free
FAB331F-025
Carrier Free
FAB331A-100
Carrier Free
FAB331A-025
Carrier Free
FAB331P-100
Carrier Free
FAB331P-025
Carrier Free
FAB331F-100
Carrier Free
FAB331N-100UG
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CXCR2/IL-8RB Antibody (48311) [Unconjugated] Summary

Additional Information
Clone 48311 was used by HLDA to establish CD designation
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB
Met1-Leu355
Accession # AAB25880
Specificity
Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CXCR2
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 1-5 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
MAB331 in the following applications:
Publications
Read Publications using
MAB331 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR2/IL-8RB Antibody (48311) [Unconjugated]

  • CD182 antigen
  • CD182
  • CDw128b
  • chemokine (C-X-C motif) receptor 2
  • chemokine (CXC) receptor 2
  • CMKAR2
  • C-X-C chemokine receptor type 2
  • CXCR2 gene for IL8 receptor type B
  • CXCR2
  • CXC-R2
  • CXCR-2
  • GRO/MGSA receptor
  • High affinity interleukin-8 receptor B
  • IL-8 RB
  • IL-8 receptor type 2
  • IL-8R B
  • IL8R2
  • IL8RA
  • IL8RB
  • interleukin 8 receptor B
  • interleukin 8 receptor type 2
  • interleukin-8 receptor type B

Background

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha , GRO beta , GRO gamma , NAP-2, and ENA-78.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR2/IL-8RB Antibody (MAB331)(66)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Guinea Pig, Rabbit.

We have publications tested in 10 applications: Bioassay, ELISA Development, Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development
(1)
Flow Cytometry
(13)
Functional Assay
(1)
ICC
(3)
IHC
(7)
IHC-Fr
(3)
IHC-P
(7)
Neutralization
(23)
Western Blot
(6)
All Applications
Filter By Species
Human
(48)
Mouse
(4)
Rat
(1)
Guinea Pig
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 66. Show All 66 Publications.
Publications using MAB331 Applications Species
Zhao, C;Wang, D;Li, Z;Zhang, Z;Xu, Y;Liu, J;Lei, Q;Han, D;Huo, Y;Liu, S;Li, L;Zhang, Y; IL8 derived from macrophages inhibits CD8+ T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancer International immunopharmacology 2023-06-16 [PMID: 37331296] (Neutralization, Human) Neutralization Human
R Tanaka, K Kimura, S Eguchi, G Ohira, S Tanaka, R Amano, H Tanaka, M Yashiro, M Ohira, S Kubo Interleukin-8 produced from cancer-associated fibroblasts suppresses proliferation of the OCUCh-LM1 cancer cell line BMC Cancer, 2022-07-08;22(1):748. 2022-07-08 [PMID: 35804329] (Neutralization, Western Blot, Human) Neutralization, Western Blot Human
G Tsujio, K Maruo, Y Yamamoto, T Sera, A Sugimoto, H Kasashima, Y Miki, M Yoshii, T Tamura, T Toyokawa, H Tanaka, K Muguruma, M Ohira, M Yashiro Significance of tumor heterogeneity of p-Smad2 and c-Met in HER2-positive gastric carcinoma with lymph node metastasis BMC Cancer, 2022-06-01;22(1):598. 2022-06-01 [PMID: 35650563] (IHC, Human) IHC Human
Y Yamamoto, A Sugimoto, K Maruo, G Tsujio, T Sera, S Kushiyama, S Nishimura, K Kuroda, S Togano, S Eguchi, R Tanaka, K Kimura, R Amano, M Ohira, M Yashiro CXCR2 signaling might have a tumor-suppressive role in patients with cholangiocarcinoma PLoS ONE, 2022-04-04;17(4):e0266027. 2022-04-04 [PMID: 35377900] (IHC, Western Blot, Human) IHC, Western Blot Human
N Freitag, DM Baston-Bue, JS Kruessel, UR Markert, TN Fehm, AP Bielfeld Eutopic endometrial immune profile of infertility-patients with and without endometriosis Journal of reproductive immunology, 2022-01-29;150(0):103489. 2022-01-29 [PMID: 35149274] (IHC, Human) IHC Human
Sinan Muldur, Douangsone D. Vadysirisack, Sharan Ragunathan, Yalan Tang, Alonso Ricardo, Camil Elie Sayegh et al. Human Neutrophils Respond to Complement Activation and Inhibition in Microfluidic Devices Frontiers in Immunology 11/24/2021 [PMID: 34899737]
Adrien Guérard, Victor Laurent, Gaëlle Fromont, David Estève, Julia Gilhodes, Edith Bonnelye et al. The Chemokine Receptor CCR3 Is Potentially Involved in the Homing of Prostate Cancer Cells to Bone: Implication of Bone-Marrow Adipocytes International Journal of Molecular Sciences 2021-02-17 [PMID: 33671469]
S Ali, TF Borin, R Piranliogl, R Ara, I Lebedyeva, K Angara, BR Achyut, AS Arbab, MH Rashid Changes in the tumor microenvironment and outcome for TME-targeting therapy in glioblastoma: A pilot study PLoS ONE, 2021-02-05;16(2):e0246646. 2021-02-05 [PMID: 33544755] (Flow Cytometry, IHC, Mouse) Flow Cytometry, IHC Mouse
Stéphane Chevrier, Yves Zurbuchen, Carlo Cervia, Sarah Adamo, Miro E. Raeber, Natalie de Souza et al. A distinct innate immune signature marks progression from mild to severe COVID-19 Cell Reports Medicine 1/1/2021 [PMID: 33521697]
AJ Hoogendijk, F Pourfarzad, CEM Aarts, ATJ Tool, IH Hiemstra, L Grassi, M Frontini, AB Meijer, M van den Bi, TW Kuijpers Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming Cell Rep, 2019-11-19;29(8):2505-2519.e4. 2019-11-19 [PMID: 31747616] (Flow Cytometry, Human) Flow Cytometry Human
Show All 66 Publications.

Reviews for CXCR2/IL-8RB Antibody (MAB331) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB331:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 IHC-P Human 02/20/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22763101
SpeciesHuman
  4
reviewed by:
Verified Customer
 IHC-P Human 02/20/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22626766
SpeciesHuman

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0
 
 Verified Customer
02/20/2015
Application: IHC-P
Species: Human
read this review
 
 Verified Customer
02/20/2015
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol CXCR2
Uniprot