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MIPEP Antibody (OTI5E7)

Images

 
Western Blot: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIPEP.
Immunohistochemistry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of Carcinoma of Human thyroid tissue.
Flow Cytometry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by MIPEP antibody.
Immunohistochemistry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of Human prostate tissue.
Immunohistochemistry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of Human pancreas tissue.
Immunohistochemistry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of Human breast tissue.
Immunohistochemistry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of Human liver tissue.
Immunohistochemistry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of Human Kidney tissue.
Immunohistochemistry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of Carcinoma of Human prostate tissue.
Immunohistochemistry: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of Human Ovary tissue.
Immunofluorescence: MIPEP Antibody (OTI5E7) [NBP2-45861] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MIPEP.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI5E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MIPEP Antibody (OTI5E7) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 174-516 of human MIPEP (NP_005923) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MIPEP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
80.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MIPEP Antibody (OTI5E7)

  • HMIP
  • MIPEC 3.4.24.59
  • mitochondrial intermediate peptidase

Background

The product of the MIPEP gene performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. This protein is primarily involved in the maturation of oxidative phosphorylation (OXPHOS)-related proteins. This gene may contribute to the functional effects of frataxin deficiency and the clinical manifestations of Friedreich ataxia. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MIPEP