![Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0006.jpg "Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA, IHC, IP, KD, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This affinity purified CEBP Delta Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse CEBP Delta. (Uniprot: Q00322) |
| Specificity | This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with CEBP Delta from human sources based on 77% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with CEBP Delta from other sources has not been determined. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CEBPD |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This affinity purified antibody has been tested for use in ELISA, western blotting, and suitable in IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kDa in size corresponding to C/EBPd by western blotting in the appropriate cell lysate or extract. The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd. |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
ICC | Human | 07/10/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for CEBP Delta Antibody (NB110-85519)Find related products by research area.
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![Western Blot: CEBP Delta Antibody [NB110-85519] - Detection of endogenous protein in whole cell extracts from mouse macrophage cells. Untreated WT cells (Lane 1), WT cells treated 3 hrs with LPS (Lane 2) and knock-out cells treated 3 hrs with LPS (Lane 3). The band at ~35 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of endogenous C/EBP delta. Primary antibody at 1:15,000. ECL was used for detection.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0001.jpg "Western Blot: CEBP Delta Antibody [NB110-85519] - Detection of endogenous protein in whole cell extracts from mouse macrophage cells. Untreated WT cells (Lane 1), WT cells treated 3 hrs with LPS (Lane 2) and knock-out cells treated 3 hrs with LPS (Lane 3). The band at ~35 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of endogenous C/EBP delta. Primary antibody at 1:15,000. ECL was used for detection.")
![Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 hrs with LPS. Lane 3: C/EBPd-knock-out cells treated 3 hrs with LPS. 35 ug per lane. Primary: anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, 35 kDa.](http://images.novusbio.com/fullsize/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0007.jpg "Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 hrs with LPS. Lane 3: C/EBPd-knock-out cells treated 3 hrs with LPS. 35 ug per lane. Primary: anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, 35 kDa.")
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