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CEBP epsilon Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446] - Staining in human bone marrow and cerebral cortex tissues using anti-CEBPE antibody. Corresponding CEBPE RNA-seq data are ...read more
Western Blot: CEBP epsilon Antibody [NBP1-85446] - Analysis in control (vector only transfected HEK293T lysate) and CEBPE over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunocytochemistry/ Immunofluorescence: CEBP epsilon Antibody [NBP1-85446] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446] - Staining of human bone marrow shows high expression.
Immunohistochemistry-Paraffin: CEBP epsilon Antibody [NBP1-85446] - Staining of human cerebral cortex shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

CEBP epsilon Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SHGTYYECEPRGGQQPLEFSGGRAGPGELGDMCEHEASIDLSAYIESGEEQLLSDLFAVKPAPEARGLKGPGTPAFPHYLPPDPRPFAYPPHTFGPDRKALGPGIYSSPGSYDPRAVAVKEEPR
Predicted Species
Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CEBPE
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CEBP epsilon Protein (NBP1-85446PEP)
Publications
Read Publications using
NBP1-85446 in the following applications:

  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31912614).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CEBP epsilon Antibody

  • C/EBP-epsilon
  • CCAAT/enhancer binding protein (C/EBP), epsilon
  • CCAAT/enhancer-binding protein epsilon
  • CEBP epsilon
  • CEBPE
  • CRP1C/EBP epsilon

Background

EBP1 (ErbB-3-binding protein 1), also known as PA2G4 (proliferation-associated 2G4), p38-2G4 or HG4-1, is a member of the peptidase M24C family and functions as an RNA-binding protein involved in cellular proliferation and differentiation processes. It is expressed in a variety of cell lines, including a wide range of tumor cell lines, and localizes to the cytoplasm. Upon treatment with Neuregulin-1 (heregulin), EBP1 translocates to the nucleus. EBP1 is a component of pre-ribosomal ribonucleoprotein complexes, participating in ribosome assembly and regulating the later steps of rRNA processing. In addition, EBP1 interacts with ErbB-3 and may function as a modulator of the ErbB-3-mediated signal transduction pathway by regulating the effects of Neuregulin-1. EBP1 also associates with histone deacetylases (HDACs), functioning as a transcriptional corepressor of cell cycle regulatory genes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CEBP epsilon Antibody (NBP1-85446)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CEBPE