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CEBP Delta Antibody [DyLight 650]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

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CEBP Delta Antibody [DyLight 650] Summary

Immunogen
This affinity purified CEBP Delta Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse CEBP Delta. (Uniprot: Q00322)
Specificity
This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with CEBP Delta from human sources based on 77% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with CEBP Delta from other sources has not been determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CEBPD
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with CEBP Delta from human sources based on 77% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with CEBP Delta from other sources has not been determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CEBP Delta Antibody [DyLight 650]

  • C/EBP-delta
  • CCAAT/enhancer binding protein (C/EBP), delta
  • CCAAT/enhancer-binding protein delta
  • CELF
  • CRP3
  • NF-IL6-betaC/EBP delta
  • Nuclear factor NF-IL6-beta

Background

C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBP delta may increase binding activity, whereas phosphorylation of the alpha and beta family members may decrease binding affinity. C/EBP delta is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CEBPD