![Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0008.jpg "Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide made to a C-terminal portion of human Factor VIII (between amino acids 2100-2250) [UniProt P00451] |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | F8 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 267 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Factor VIII Antibody (NB100-91761)Find related products by research area.
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Factor VIII - a key factor in the clotting process Hemostasis, or blood clotting, follows tissue injury and involves the deployment of essential plasma procoagulants (such as prothrombin, and Factors X, IX, V, and VIII) that trigger the blood coagulation cascade. This cascade leads to the formation... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91761-img0005.jpg "Immunocytochemistry/Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.")
![Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - Staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and the detection was performed using DAB with hematoxylin counterstaining.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Immunohistochemistry-Paraffin-NB100-91761-img0007.jpg "Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - Staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and the detection was performed using DAB with hematoxylin counterstaining.")
![Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of human placenta tissue lysate using Factor VIII antibody at 1:500.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0002.jpg "Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of human placenta tissue lysate using Factor VIII antibody at 1:500.")
![Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of HepG2 whole cell lysate (lane 1), Mouse liver tissue lysate (lane 2), and Rat liver tissue lysate (lane 3) using Factor VIII antibody at 1:500 with ECL based detection.](http://images.novusbio.com/fullsize/Factor-VIII-Antibody-Western-Blot-NB100-91761-img0006.jpg "Western Blot: Factor VIII Antibody [NB100-91761] - Analysis of HepG2 whole cell lysate (lane 1), Mouse liver tissue lysate (lane 2), and Rat liver tissue lysate (lane 3) using Factor VIII antibody at 1:500 with ECL based detection.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")