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Endoglin/CD105 Antibody [Unconjugated]

Images

 
Western blot shows lysates of bEnd.3 mouse endothelioma cell line and MS-1 mouse pancreatic islet endothelial cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen ...read more
Endoglin/CD105 was detected in immersion fixed MS-1 mouse pancreatic islet endothelial cell line using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 ...read more
Endoglin/CD105 was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room ...read more
Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (E13-15) using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 15 µg/mL overnight at 4 ...read more
Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Endoglin/CD105 at approximately 121 kDa (as indicated) using 5 µg/mL of Goat ...read more
Chronic intermittent hypoxia modifies the BM vascular structure. a’–e’ Representative images of femur bone marrow stained with vWF, CD105, VE-cadherin, SMA, and CD11b counterstained with hematoxylin. a”, c”, ...read more
Increased tumor necrosis and reduced tumor vascularization after GET of therapeutic plasmids in vivo.Histologically stained and analyzed sections after treatments of TS/A tumors: intratumoral injection of endotoxin-free ...read more
Increased tumor necrosis and reduced tumor vascularization after GET of therapeutic plasmids in vivo.Histologically stained and analyzed sections after treatments of TS/A tumors: intratumoral injection of endotoxin-free ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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Endoglin/CD105 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105
Glu27-Gly581
Accession # Q8K100
Specificity
Detects mouse Endoglin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant rat Endoglin is observed and less than 5% cross-reactivity with recombinant human Endoglin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ENG
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF1320 in the following applications:

Publications
Read Publications using
AF1320 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endoglin/CD105 Antibody [Unconjugated]

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Background

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

  1. Ge, A.Z. and E.C. Butcher (1994) Gene 138:201.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endoglin/CD105 Antibody (AF1320)(44)

We have publications tested in 4 confirmed species: Human, Mouse, Transgenic Mouse, Xenograft.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Immunohistochemistry, Western Blot.


Filter By Application
ICC
(1)
IHC
(12)
IHC-Fr
(1)
IHC-P
(4)
Immunohistochemistry
(2)
Western Blot
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(16)
Transgenic Mouse
(1)
Xenograft
(1)
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Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using AF1320 Applications Species
Shabani, Z;Do Prado, LB;Zhang, R;Zhu, W;Shaligram, SS;Yadav, A;Wang, C;Su, H; Increasing Endoglin Deletion in Endothelial Cells Exacerbates the Severity of Brain Arteriovenous Malformation in Mouse Biomedicines 2024-07-30 [PMID: 39200156] (Immunohistochemistry, Western Blot, Transgenic Mouse) Immunohistochemistry, Western Blot Transgenic Mouse
Becker, IC;Wilkie, AR;Nikols, E;Carminita, E;Roweth, HG;Tilburg, J;Sciaudone, AR;Noetzli, LJ;Fatima, F;Couldwell, G;Ray, A;Mogilner, A;Machlus, KR;Italiano, JE; Cell cycle-dependent centrosome clustering precedes proplatelet formation Science advances 2024-06-21 [PMID: 38896608] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Bram Callewaert, Willy Gsell, Uwe Himmelreich, Elizabeth A. V. Jones Q-VAT: Quantitative Vascular Analysis Tool Frontiers in Cardiovascular Medicine 2023-06-02 [PMID: 37332588]
Soojung Lee, Nicolai J. Toft, Trine V. Axelsen, Maria Sofia Espejo, Tina M. Pedersen, Marco Mele et al. Carbonic anhydrases reduce the acidity of the tumor microenvironment, promote immune infiltration, decelerate tumor growth, and improve survival in ErbB2/HER2-enriched breast cancer Breast Cancer Research 2023-04-25 [PMID: 37098526]
Madhurima Paul, Shreeta Chakraborty, Safirul Islam, Rupasri Ain Trans-differentiation of trophoblast stem cells: implications in placental biology Life Science Alliance 2023-03-01 [PMID: 36574992]
José I. Erices, Ignacio Niechi, Atenea Uribe-Ojeda, María de los Ángeles Toro, Noemí García-Romero, Josefa Carrión-Navarro et al. The low affinity A2B adenosine receptor enhances migratory and invasive capacity in vitro and angiogenesis in vivo of glioblastoma stem-like cells Frontiers in Oncology 2022-08-18 [PMID: 36059665]
S Magalhaes-, J Blecha, R Naraine, J Mikesova, P Abaffy, A Pecinova, M Milosevic, R Bohuslavov, J Prochazka, S Khan, E Novotna, R Sindelka, R Machan, M Dewerchin, E Vlcak, J Kalucka, S Stemberkov, A Benda, J Goveia, T Mracek, C Barinka, P Carmeliet, J Neuzil, K Rohlenova, J Rohlena Mitochondrial respiration supports autophagy to provide stress resistance during quiescence Autophagy, 2022-03-08;0(0):1-18. 2022-03-08 [PMID: 35258392] (Western Blot, Human) Western Blot Human
Jacinta I. Kalisch-Smith, Emily C. Morris, Mary A. A. Strevens, Andia N. Redpath, Kostantinos Klaourakis, Dorota Szumska et al. Analysis of Placental Arteriovenous Formation Reveals New Insights Into Embryos With Congenital Heart Defects Frontiers in Genetics 2022-01-19 [PMID: 35126469]
Raquel F Videira, Anne Marie C Koop, Lara Ottaviani, Ella M Poels, Jordy M M Kocken, Cristobal Dos Remedios et al. The adult heart requires baseline expression of the transcription factor Hand2 to withstand right ventricular pressure overload Cardiovascular Research 2022-09-20 [PMID: 34550326]
Jessica Cristina Marín-Llera, Carlos Ignacio Lorda-Diez, Juan Mario Hurle, Jesús Chimal-Monroy SCA-1/Ly6A Mesodermal Skeletal Progenitor Subpopulations Reveal Differential Commitment of Early Limb Bud Cells Frontiers in Cell and Developmental Biology 2021-07-16 [PMID: 34336823]
Show All 44 Publications.

Reviews for Endoglin/CD105 Antibody (AF1320) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF1320:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23593103
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 21060150
SpeciesMouse

Product General Protocols

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FAQs for Endoglin/CD105 Antibody (AF1320). (Showing 1 - 2 of 2 FAQs).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
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Verified Customer
01/05/2015
Application: IF
Species: Mouse

 
Verified Customer
01/05/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol ENG
Uniprot