Home » CD25/IL-2R alpha » CD25/IL-2R alpha Antibodies » CD25/IL-2R alpha Antibody (OX-39)
Novus Biologicals products are now on bio-techne.com

CD25/IL-2R alpha Antibody (OX-39)

 (4 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
Immunocytochemistry/ Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8, green in B. C is the merged ...read more
Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection ...read more
Flow Cytometry: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin
Immunocytochemistry/ Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 antibody, red in A and Mouse anti Rat CD8, green in B. C is the merged ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA, Flow, IHC, IP
Clone
OX-39
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-64761AF350
Alexa Fluor 405
NB100-64761AF405
Alexa Fluor 488
NB100-64761AF488
Alexa Fluor 532
NB100-64761AF532
Alexa Fluor 594
NB100-64761AF594
Alexa Fluor 647
NB100-64761AF647
Alexa Fluor 700
NB100-64761AF700
Alexa Fluor 750
NB100-64761AF750
Allophycocyanin
NB100-64761APC
Allophycocyanin/Cy7
NB100-64761APCCY7
Biotin
NB100-64761B
CoraFluor 1
NB100-64761CL1
DyLight 350
NB100-64761UV
DyLight 405
NB100-64761V
DyLight 488
NB100-64761G
DyLight 550
NB100-64761R
DyLight 594
NB100-64761DL594
DyLight 650
NB100-64761C
DyLight 680
NB100-64761FR
DyLight 755
NB100-64761IR
FITC
NB100-64761F
HRP
NB100-64761H
Janelia Fluor 525
NB100-64761JF525
Janelia Fluor 549
NB100-64761JF549
Janelia Fluor 646
NB100-64761JF646
Janelia Fluor 669
NB100-64761JF669
PE
NB100-64761PE
PE/Atto594
NB100-64761PEATT594
PE/Cy5.5
NB100-64761PECY55
PE/Cy7
NB100-64761PECY7
PerCP
NB100-64761PCP
mFluor Violet 450 SE
NB100-64761MFV450
mFluor Violet 500 SE
NB100-64761MFV500
mFluor Violet 610 SE
NB100-64761MFV610
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

CD25/IL-2R alpha Antibody (OX-39) Summary

Immunogen
Stimulated Rat T cells
Localization
Cell Membrane
Specificity
NB100-64761 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) ^(6). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL2RA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Publications
Read Publications using
NB100-64761 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD25/IL-2R alpha Antibody (OX-39)

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen
  • TCGFR

Background

The IL2 receptor (IL2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220 low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220 low TdT+ sIg- pro-B/pre B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T cell development in fetal and adult thymus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
FoxP3 Antibody -
BSA Free
NB100-39002
Flow Cytometry FoxP3 Antibody - BSA Free
Flow Cytometry FoxP3 Antibody - BSA Free
Immunohistochemistry-Paraffin FoxP3 Antibody - BSA Free
(2 Reviews)
(62 Publications)
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(303 Publications)
Species: Mu
Applications: ELISA
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
CD3 Antibody
(17A2) [Unconjugated]
MAB4841
Immunohistochemistry CD3 Antibody (17A2) [Unconjugated]
Immunocytochemistry CD3 Antibody (17A2) [Unconjugated]
Immunohistochemistry CD3 Antibody (17A2) [Unconjugated]
(42 Publications)
Species: Mu
Applications: CompCytotox, CyTOF-ready, Flow, ICC, IHC, IP, Tstim
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(455 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(670 Publications)
Species: Mu
Applications: ELISA
CD69 Antibody
[Unconjugated]
AF2386
Flow Cytometry CD69 Antibody [Unconjugated]
(5 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, WB
IL-2 R beta
Antibody (27302)
MAB224
Immunohistochemistry IL-2 R beta Antibody (27302)
Neutralization IL-2 R beta Antibody (27302)
(7 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
TNF RII/TNFRSF1B
[HRP]
DRT200
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
(76 Publications)
Species: Hu
Applications: ELISA
CDP/CUTL1 Antibody
(2A10)
H00001523-M01
Western Blot CDP/CUTL1 Antibody (2A10)
Immunohistochemistry-Paraffin CDP/CUTL1 Antibody (2A10)
ELISA CDP/CUTL1 Antibody (2A10)
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
LEDGF Antibody
[Unconjugated]
AF3468
Western Blot LEDGF Antibody [Unconjugated]
Simple Western LEDGF Antibody [Unconjugated]
Immunocytochemistry LEDGF Antibody [Unconjugated]
Species: Hu
Applications: ICC, KO, Simple Western, WB
HCLS1 Antibody
(1A8)
H00003059-M02
Western Blot HCLS1 Antibody (1A8)
Proximity Ligation Assay HCLS1 Antibody (1A8)
Immunohistochemistry-Paraffin HCLS1 Antibody (1A8)
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, PLA, S-ELISA, WB
KHSRP Antibody
NBP1-18910
Immunohistochemistry-Paraffin KHSRP Antibody
Immunohistochemistry-Paraffin KHSRP Antibody
Western Blot KHSRP Antibody
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB

Publications for CD25/IL-2R alpha Antibody (NB100-64761)(4)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: FLOW, IHC-Fr.


Filter By Application
FLOW
(2)
IHC-Fr
(1)
All Applications
Filter By Species
Rat
(2)
All Species

Reviews for CD25/IL-2R alpha Antibody (NB100-64761) (0)

There are no reviews for CD25/IL-2R alpha Antibody (NB100-64761). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD25/IL-2R alpha Antibody (NB100-64761) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CD25/IL-2R alpha Products

Research Areas for CD25/IL-2R alpha Antibody (NB100-64761)

Find related products by research area.

Blogs on CD25/IL-2R alpha

There are no specific blogs for CD25/IL-2R alpha, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CD25/IL-2R alpha Antibody (OX-39) and receive a gift card or discount.


Bioinformatics

Gene Symbol IL2RA
Entrez
Uniprot