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LEDGF Antibody [Unconjugated]

 
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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and human CD4+T cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEDGF p75 at approximately 75 kDa and LEDGF p52 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and human CD4+T cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
LEDGF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) at 10 µg/mL for 3 hours at room ...read more
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LEDGF p75 at ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LEDGF knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3468-SP
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LEDGF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human LEDGF
Met1-Asn530
Accession # O75475
Specificity
Detects human LEDGF in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PSIP1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LEDGF Antibody [Unconjugated]

  • CLL-associated antigen KW-7
  • Dense fine speckles 70 kDa protein
  • DFS 70
  • DFS70
  • LEDGF
  • LEDGFDFS 70
  • Lens epithelium-derived growth factor
  • MGC74712
  • p52
  • p75
  • PAIP
  • PC4 and SFRS1 interacting protein 1
  • PC4 and SFRS1 interacting protein 2
  • PC4 and SFRS1-interacting protein
  • PSIP1
  • PSIP2
  • transcriptional coactivator p52/p75
  • Transcriptional coactivator p75/p52

Background

LEDGF, also known as transcription co-activator p75, is a 75 kDa protein that belongs to the hepatoma-derived growth factor (HDGF) family of nuclear targeted proteins. It is a widely expressed protein that interacts with lentiviral integrase. As a result of alternative splicing, isoforms with deletions, substitutions/insertions have been reported. Human LEDGF shares 91%, 92% and 96% amino acid sequence homology with rat, mouse and feline LEDGF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol PSIP1
Uniprot