Home » CD25/IL-2R alpha » CD25/IL-2R alpha Antibodies » CD25/IL-2R alpha Antibody (OX-39) [CoraFluor™ 1]
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CD25/IL-2R alpha Antibody (OX-39) [CoraFluor™ 1]

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, IP
Clone
OX-39
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-64761AF350
Alexa Fluor 405
NB100-64761AF405
Alexa Fluor 488
NB100-64761AF488
Alexa Fluor 532
NB100-64761AF532
Alexa Fluor 594
NB100-64761AF594
Alexa Fluor 647
NB100-64761AF647
Alexa Fluor 700
NB100-64761AF700
Alexa Fluor 750
NB100-64761AF750
Allophycocyanin
NB100-64761APC
Allophycocyanin/Cy7
NB100-64761APCCY7
Biotin
NB100-64761B
DyLight 350
NB100-64761UV
DyLight 405
NB100-64761V
DyLight 488
NB100-64761G
DyLight 550
NB100-64761R
DyLight 594
NB100-64761DL594
DyLight 650
NB100-64761C
DyLight 680
NB100-64761FR
DyLight 755
NB100-64761IR
FITC
NB100-64761F
HRP
NB100-64761H
Janelia Fluor 525
NB100-64761JF525
Janelia Fluor 549
NB100-64761JF549
Janelia Fluor 646
NB100-64761JF646
Janelia Fluor 669
NB100-64761JF669
PE
NB100-64761PE
PE/Atto594
NB100-64761PEATT594
PE/Cy5.5
NB100-64761PECY55
PE/Cy7
NB100-64761PECY7
PerCP
NB100-64761PCP
Unconjugated
NB100-64761
mFluor Violet 450 SE
NB100-64761MFV450
mFluor Violet 500 SE
NB100-64761MFV500
mFluor Violet 610 SE
NB100-64761MFV610
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CD25/IL-2R alpha Antibody (OX-39) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Stimulated Rat T cells
Localization
Cell Membrane
Specificity
NB100-64761 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) ^(6). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL2RA
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CD25/IL-2R alpha Antibody (OX-39) [CoraFluor™ 1]

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen
  • TCGFR

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL2RA