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CD25/IL-2R alpha Antibodies

Cluster of Differentiation 25 (CD25), also called Interleukin-2 Receptor (IL-2R) alpha, is a type I transmembrane protein that associates with IL-2R beta and gamma to form a heterotrimeric receptor for IL-2. CD25/IL-2R alpha is expressed on the surface of immune cells and plays a role in regulatory T (Treg) cell function and immune tolerance. The human IL-2R alpha protein is 272 amino acids (aa) in length with a theoretical molecular weight (MW) of 30.8 kDa. Our CD25/IL-2R alpha antibodies have been cited in 25+ publications. Browse our catalog for recombinant monoclonal, monoclonal, polyclonal, and IL-2R alpha-conjugated antibodies. Validated research applications for these CD25/IL-2R alpha antibodies include Blocking/Neutralizing, CyTOF-ready, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, and Western Blot. Additionally, we offer a knockout (KO) validated antibody option. Our CD25/IL-2R alpha antibodies are reactive in Human, Mouse, Rat, Primate, and more. Select the anti-IL-2R alpha antibody best suited for your research needs by viewing the technical details, images, references, and related products. Each antibody is fully covered by Novus’ Guarantee+ to give you peace of mind and support when you need it.
Alternate Names for CD25/IL-2R alpha Antibodies
anti-CD25 antigen antibody, anti-CD25 antibody, anti-IDDM10 antibody, anti-IL-2 R alpha antibody, anti-IL-2 receptor subunit alpha antibody, anti-IL2R alpha antibody, anti-IL-2R subunit alpha antibody, anti-IL2R antibody, anti-IL2RA antibody, anti-IL-2Ra antibody, anti-IL-2-RA antibody, anti-IL2-RA antibody, anti-interleukin 2 receptor, alpha antibody, anti-interleukin-2 receptor subunit alpha antibody, anti-p55 antibody, anti-TAC antigen antibody, anti-TCGFR antibody
45 Results for "cd25-il-2r-alpha" in Primary Antibodies
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NB600-710

CD25/IL-2R alpha Antibody (PC61.5.3) - BSA Free

0.25 mg
NB600-710
Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (PC61.5.3) [NB600-710] - Analysis of formalin-fixed paraffin-embedded tissue section of spleen from normal Mouse using CD25/IL-2 R alpha antibody (clone PC61.5.3.) at 1:100 dilution.
Mu
Species Glossary
Reactivity: Mu
Flow, IHC, IP, CyTOF-ready
Applications: Flow, IHC, IP, CyTOF-ready
Rat Monoclonal
Host: Rat Monoclonal
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NB600-710AF350
Alexa Fluor 405 NB600-710AF405
Alexa Fluor 488 NB600-710AF488
Alexa Fluor 532 NB600-710AF532
Alexa Fluor 594 NB600-710AF594
Alexa Fluor 647 NB600-710AF647
Alexa Fluor 700 NB600-710AF700
Alexa Fluor 750 NB600-710AF750
Allophycocyanin NB600-710APC
Biotin NB600-710B
DyLight 350 NB600-710UV
DyLight 405 NB600-710V
DyLight 488 NB600-710G
DyLight 550 NB600-710R
DyLight 594 NB600-710DL594
DyLight 650 NB600-710C
DyLight 680 NB600-710FR
DyLight 755 NB600-710IR
FITC NB600-710F
HRP NB600-710H
Janelia Fluor 525 NB600-710JF525
Janelia Fluor 549 NB600-710JF549
Janelia Fluor 585 NB600-710JF585
Janelia Fluor 635 NB600-710JF635
Janelia Fluor 646 NB600-710JF646
Janelia Fluor 669 NB600-710JF669
PE NB600-710PE
PerCP NB600-710PCP
Available Formulations
Formulation Catalog # Availability Price  
Azide and BSA Free NBP2-80637
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MAB623

CD25/IL-2R alpha Antibody (24204) [Unconjugated]

(6 Publications)
100 μg
MAB623
Western blot shows 25 ng of Recombinant Human CD25/IL-2 R alpha (Catalog # 223-2A), Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM) and Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB623) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD25/IL-2 R alpha at approximately 30-250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.
Hu
Species Glossary
Reactivity: Hu
WB, IHC, ELISA(Cap), ICC/IF
Applications: WB, IHC, ELISA(Cap), ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB623-SP
Carrier Free MAB623-500
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MAB223

CD25/IL-2R alpha Antibody (22722) [Unconjugated]

(8 Publications)
100 μg
MAB223
CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB223) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Hu
Species Glossary
Reactivity: Hu
WB, B/N, ICC/IF
Applications: WB, B/N, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB223-SP
Carrier Free MAB223-500
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AF-223-NA

CD25/IL-2R alpha Antibody [Unconjugated]

(6 Publications)
100 μg
AF-223-NA
Western blot shows lysates of HDLM-2 human Hodgkin's lymphoma cell line and PC-3 human prostate cancer cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD25/IL-2R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated). GAPDH (AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Flow, B/N, CyTOF-ready, KO
Applications: WB, Flow, B/N, CyTOF-ready, KO
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF223
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF-223-SP
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AF2438

CD25/IL-2R alpha Antibody [Unconjugated]

(3 Publications)
100 μg
AF2438
Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CD25/IL‑2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438). The ND50 is typically 5-20 µg/mL.
Mu
Species Glossary
Reactivity: Mu
WB, IHC, B/N, ELISA(Cap)
Applications: WB, IHC, B/N, ELISA(Cap)
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF2438
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF2438-SP
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MAB1020

CD25/IL-2R alpha Antibody (24212) [Unconjugated]

(5 Publications)
500 μg
MAB1020
In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 μg/mL of Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB1020). Mouse IgG2A Isotype Control (Catalog # MAB003) at 0.5 μg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).
Hu
Species Glossary
Reactivity: Hu
WB, Flow, B/N, CyTOF-ready
Applications: WB, Flow, B/N, CyTOF-ready
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB1020U-100UG
Alexa Fluor 405 FAB1020V-100UG
Alexa Fluor 488 FAB1020G-100UG
Alexa Fluor 532 FAB1020X
Alexa Fluor 594 FAB1020T-100UG
Alexa Fluor 647 FAB1020R-100UG
Alexa Fluor 700 FAB1020N-100UG
Alexa Fluor 750 FAB1020S-100UG
Allophycocyanin FAB1020A-025
Allophycocyanin FAB1020A-100
Allophycocyanin/Cy7 FAB1020APCCY7
Biotin FAB1020B
DyLight 350 FAB1020D
DyLight 405 FAB1020E
DyLight 488 FAB1020K
DyLight 550 FAB1020L
DyLight 594 FAB1020M
DyLight 650 FAB1020W
DyLight 680 FAB1020Y
DyLight 755 FAB1020Z
FITC FAB1020F
HRP FAB1020H
Janelia Fluor 525 FAB1020JF525
Janelia Fluor 549 FAB1020I
Janelia Fluor 585 FAB1020JF585
Janelia Fluor 635 FAB1020JF635
Janelia Fluor 646 FAB1020J
Janelia Fluor 669 FAB1020JF669
PE/Atto594 FAB1020PEATT594
PE/Cy5.5 FAB1020PECY55
PE/Cy7 FAB1020PECY7
PerCP FAB1020C
Phycoerythrin FAB1020P-100
Phycoerythrin FAB1020P-025
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free FAB1020G-100UG
Carrier Free FAB1020P-100
Carrier Free FAB1020A-100
Carrier Free FAB1020A-025
Carrier Free FAB1020P-025
Carrier Free MAB1020-SP
Carrier Free FAB1020R-100UG
Carrier Free FAB1020N-100UG
Carrier Free FAB1020V-100UG
Carrier Free FAB1020U-100UG
Carrier Free FAB1020T-100UG
Carrier Free FAB1020S-100UG
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NBP2-37349

CD25/IL-2R alpha Antibody (1B5D12) - BSA Free

0.1 ml
NBP2-37349
Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.
Hu, Mu, Rt, Pm, Pm
Species Glossary
Reactivity: Hu, Mu, Rt, Pm, Pm
WB, ELISA, ICC/IF, IHC
Applications: WB, ELISA, ICC/IF, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
BSA Free NBP2-37349-0.025ml
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NB100-64761

CD25/IL-2R alpha Antibody (OX-39)

(4 Publications)
0.25 mg
NB100-64761
Immunocytochemistry/Immunofluorescence: CD25/IL-2 R alpha Antibody (OX-39) [NB100-64761] - Rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
Rt
Species Glossary
Reactivity: Rt
ELISA, Flow, IHC, IP
Applications: ELISA, Flow, IHC, IP
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NB100-64761AF350
Alexa Fluor 405 NB100-64761AF405
Alexa Fluor 488 NB100-64761AF488
Alexa Fluor 532 NB100-64761AF532
Alexa Fluor 594 NB100-64761AF594
Alexa Fluor 647 NB100-64761AF647
Alexa Fluor 700 NB100-64761AF700
Alexa Fluor 750 NB100-64761AF750
Allophycocyanin NB100-64761APC
Allophycocyanin/Cy7 NB100-64761APCCY7
Biotin NB100-64761B
CoraFluor 1 NB100-64761CL1
DyLight 350 NB100-64761UV
DyLight 405 NB100-64761V
DyLight 488 NB100-64761G
DyLight 550 NB100-64761R
DyLight 594 NB100-64761DL594
DyLight 650 NB100-64761C
DyLight 680 NB100-64761FR
DyLight 755 NB100-64761IR
FITC NB100-64761F
HRP NB100-64761H
Janelia Fluor 525 NB100-64761JF525
Janelia Fluor 549 NB100-64761JF549
Janelia Fluor 585 NB100-64761JF585
Janelia Fluor 635 NB100-64761JF635
Janelia Fluor 646 NB100-64761JF646
Janelia Fluor 669 NB100-64761JF669
PE NB100-64761PE
PE/Atto594 NB100-64761PEATT594
PE/Cy5.5 NB100-64761PECY55
PE/Cy7 NB100-64761PECY7
PerCP NB100-64761PCP
mFluor Violet 450 SE NB100-64761MFV450
mFluor Violet 500 SE NB100-64761MFV500
mFluor Violet 610 SE NB100-64761MFV610
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MAB9926

CD25/IL-2R alpha Antibody (Hu107) [Unconjugated]

100 μg
MAB9926
In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 μg/mL of Human Anti-Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Monoclonal Antibody (Catalog # MAB9926). Human IgG Isotype Control (Catalog # 1-001-A) at 0.5 μg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).
Hu
Species Glossary
Reactivity: Hu
Flow, B/N, CyTOF-ready
Applications: Flow, B/N, CyTOF-ready
Human Monoclonal
Host: Human Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB9926U-100UG
Alexa Fluor 405 FAB9926V-100UG
Alexa Fluor 488 FAB9926G-100UG
Alexa Fluor 532 FAB9926X
Alexa Fluor 594 FAB9926T-100UG
Alexa Fluor 647 FAB9926R-100UG
Alexa Fluor 700 FAB9926N-100UG
Alexa Fluor 750 FAB9926S-100UG
Allophycocyanin FAB9926A
Allophycocyanin/Cy7 FAB9926APCCY7
Biotin FAB9926B
CoraFluor 1 FAB9926CL1
DyLight 350 FAB9926D
DyLight 405 FAB9926E
DyLight 488 FAB9926K
DyLight 550 FAB9926L
DyLight 594 FAB9926M
DyLight 650 FAB9926W
DyLight 680 FAB9926Y
DyLight 755 FAB9926Z
FITC FAB9926F
HRP FAB9926H
Janelia Fluor 525 FAB9926JF525
Janelia Fluor 549 FAB9926I
Janelia Fluor 585 FAB9926JF585
Janelia Fluor 635 FAB9926JF635
Janelia Fluor 646 FAB9926J
Janelia Fluor 669 FAB9926JF669
PE FAB9926P
PE/Atto594 FAB9926PEATT594
PE/Cy5.5 FAB9926PECY55
PE/Cy7 FAB9926PECY7
PerCP FAB9926C
mFluor Violet 450 SE FAB9926MFV450
mFluor Violet 500 SE FAB9926MFV500
mFluor Violet 610 SE FAB9926MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB9926-SP
Carrier Free FAB9926V-100UG
Carrier Free FAB9926U-100UG
Carrier Free FAB9926T-100UG
Carrier Free FAB9926S-100UG
Carrier Free FAB9926R-100UG
Carrier Free FAB9926N-100UG
Carrier Free FAB9926G-100UG
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MAB9927

CD25/IL-2R alpha Antibody (Hu102) [Unconjugated]

(1 Publication)
100 μg
MAB9927
Human PBMCs were treated with PHA (5 µg/mL for 24hr) then stained with (A) Human CD25/IL-2 Ra (Daclizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9927) or (B) Normal Human IgG control antibody (Catalog # 1-001-A), followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antiobdy (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
Hu
Species Glossary
Reactivity: Hu
Flow, CyTOF-ready
Applications: Flow, CyTOF-ready
Human Monoclonal
Host: Human Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB9927U-100UG
Alexa Fluor 405 FAB9927V-100UG
Alexa Fluor 488 FAB9927G-100UG
Alexa Fluor 532 FAB9927X
Alexa Fluor 594 FAB9927T-100UG
Alexa Fluor 647 FAB9927R-100UG
Alexa Fluor 700 FAB9927N-100UG
Alexa Fluor 750 FAB9927S-100UG
Allophycocyanin FAB9927A
Allophycocyanin/Cy7 FAB9927APCCY7
Biotin FAB9927B
CoraFluor 1 FAB9927CL1
DyLight 350 FAB9927D
DyLight 405 FAB9927E
DyLight 488 FAB9927K
DyLight 550 FAB9927L
DyLight 594 FAB9927M
DyLight 650 FAB9927W
DyLight 680 FAB9927Y
DyLight 755 FAB9927Z
FITC FAB9927F
HRP FAB9927H
Janelia Fluor 525 FAB9927JF525
Janelia Fluor 549 FAB9927I
Janelia Fluor 585 FAB9927JF585
Janelia Fluor 635 FAB9927JF635
Janelia Fluor 646 FAB9927J
Janelia Fluor 669 FAB9927JF669
PE FAB9927P
PE/Atto594 FAB9927PEATT594
PE/Cy5.5 FAB9927PECY55
PE/Cy7 FAB9927PECY7
PerCP FAB9927C
mFluor Violet 450 SE FAB9927MFV450
mFluor Violet 500 SE FAB9927MFV500
mFluor Violet 610 SE FAB9927MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB9927-SP
Carrier Free FAB9927V-100UG
Carrier Free FAB9927U-100UG
Carrier Free FAB9927T-100UG
Carrier Free FAB9927S-100UG
Carrier Free FAB9927R-100UG
Carrier Free FAB9927N-100UG
Carrier Free FAB9927G-100UG
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AB-223-NA

CD25/IL-2R alpha Antibody

(1 Publication)
1 mg
AB-223-NA
Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL‑2 R alpha Polyclonal Antibody (Catalog # AB-223-NA). The ND50 is typically 5-20 µg/mL.
Hu
Species Glossary
Reactivity: Hu
WB, B/N
Applications: WB, B/N
Goat Polyclonal
Host: Goat Polyclonal
Unconjugated  
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NBP2-52661

CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric - Azide and BSA Free

0.2 mg
NBP2-52661
Flow Cytometry: CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric [NBP2-52661] - Flow-cytometry using anti-CD25 antibody Basiliximab (NBP2-52661) Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Basiliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Hu, Pm-Cm, RM
Species Glossary
Reactivity: Hu, Pm-Cm, RM
WB, Flow, ICC/IF, IHC, B/N, CyTOF-ready
Applications: WB, Flow, ICC/IF, IHC, B/N, CyTOF-ready
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NBP2-52661AF350
Alexa Fluor 405 NBP2-52661AF405
Alexa Fluor 488 NBP2-52661AF488
Alexa Fluor 532 NBP2-52661AF532
Alexa Fluor 594 NBP2-52661AF594
Alexa Fluor 647 NBP2-52661AF647
Alexa Fluor 700 NBP2-52661AF700
Alexa Fluor 750 NBP2-52661AF750
Allophycocyanin NBP2-52661APC
Biotin NBP2-52661B
DyLight 350 NBP2-52661UV
DyLight 405 NBP2-52661V
DyLight 488 NBP2-52661G
DyLight 550 NBP2-52661R
DyLight 594 NBP2-52661DL594
DyLight 650 NBP2-52661C
DyLight 680 NBP2-52661FR
DyLight 755 NBP2-52661IR
FITC NBP2-52661F
HRP NBP2-52661H
Janelia Fluor 549 NBP2-52661JF549
Janelia Fluor 646 NBP2-52661JF646
PE NBP2-52661PE
PE/Atto594 NBP2-52661PEATT594
PE/Cy5.5 NBP2-52661PECY55
PE/Cy7 NBP2-52661PECY7
PerCP NBP2-52661PCP
mFluor Violet 450 SE NBP2-52661MFV450
mFluor Violet 500 SE NBP2-52661MFV500
mFluor Violet 610 SE NBP2-52661MFV610
Available Formulations
Formulation Catalog # Availability Price  
Azide and BSA Free NBP2-52661-0.025mg
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