![Western Blot: BubR1 Antibody (8G1) - Azide and BSA Free [NBP2-80606] - BUBR1 detected in HeLa cell lysate using NB 100-353. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Azide-and-BSA-Free-Western-Blot-NBP2-80606-img0001.jpg "Western Blot: BubR1 Antibody (8G1) - Azide and BSA Free [NBP2-80606] - BUBR1 detected in HeLa cell lysate using NB 100-353. Image from the standard format of this antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IP, CyTOF-ready |
| Clone | 8G1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350). [UniProt# O60566] |
| Localization | Nuclear |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BUB1B |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This BubR1 (8G1) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot. By WB, this antibody recognizes a band at ~120 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready. |
| Theoretical MW | 120 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BubR1 Antibody (NBP2-80606)Find related products by research area.
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| Gene Symbol | BUB1B |
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) - Azide and BSA Free [NBP2-80606] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80606-img0002.jpg "Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) - Azide and BSA Free [NBP2-80606] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK. Image from the standard format of this antibody.")
![Flow Cytometry: BubR1 Antibody (8G1) - Azide and BSA Free [NBP2-80606] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Dylight 650.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80606-img0003.jpg "Flow Cytometry: BubR1 Antibody (8G1) - Azide and BSA Free [NBP2-80606] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Dylight 650.")
![Flow Cytometry: BubR1 Antibody (8G1) - Azide and BSA Free [NBP2-80606] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80606-img0004.jpg "Flow Cytometry: BubR1 Antibody (8G1) - Azide and BSA Free [NBP2-80606] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")