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BubR1 Antibody (8G1) [Janelia Fluor® 585]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IP, CyTOF-ready
Clone
8G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 585

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-353AF350
Alexa Fluor 405
NB100-353AF405
Alexa Fluor 488
NB100-353AF488
Alexa Fluor 532
NB100-353AF532
Alexa Fluor 594
NB100-353AF594
Alexa Fluor 647
NB100-353AF647
Alexa Fluor 700
NB100-353AF700
Alexa Fluor 750
NB100-353AF750
Allophycocyanin
NB100-353APC
Biotin
NB100-353B
CoraFluor 1
NB100-353CL1
DyLight 350
NB100-353UV
DyLight 405
NB100-353V
DyLight 488
NB100-353G
DyLight 550
NB100-353R
DyLight 594
NB100-353DL594
DyLight 650
NB100-353C
DyLight 680
NB100-353FR
DyLight 755
NB100-353IR
FITC
NB100-353F
HRP
NB100-353H
Janelia Fluor 525
NB100-353JF525
Janelia Fluor 549
NB100-353JF549
Janelia Fluor 635
NB100-353JF635
Janelia Fluor 646
NB100-353JF646
Janelia Fluor 669
NB100-353JF669
PE
NB100-353PE
Unconjugated
NB100-353
mFluor Violet 450 SE
NB100-353MFV450
mFluor Violet 500 SE
NB100-353MFV500
mFluor Violet 610 SE
NB100-353MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-353
BSA Free
NB100-353
BSA Free
NB100-353SS
Azide and BSA Free
NBP2-80606
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BubR1 Antibody (8G1) [Janelia Fluor® 585] Summary

Immunogen
A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350). [UniProt# O60566]
Specificity
NB100-353 is specific for hBUBR1. There should be no cross-reactivity with hBUB1.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BUB1B
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for BubR1 Antibody (8G1) [Janelia Fluor® 585]

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC 2.7.11.1
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.

BubR1 antibodies are useful tools for cancer research and cell division studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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BubR1 Antibody
(8G1) [Janelia Fluor& ...
NB100-353JF585
BubR1 Antibody (8G1) [Janelia Fluor® 585]
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BUB1B