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![Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Western-Blot-NB100-353-img0006.jpg "Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Immunocytochemistry-Immunofluorescence-NB100-353-img0005.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0008.jpg)
![Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using BubR1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BubR1-Antibody-8G1-Flow-Cytometry-NB100-353-img0007.jpg)
![Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Western-Blot-NBP1-47680-img0002.jpg "Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.")
![Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) [NBP1-47680] - BubR1 Antibody (6E5) [NBP1-47680] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunocytochemistry-Immunofluorescence-NBP1-47680-img0012.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0009.jpg)
![Flow Cytometry: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells transfected with either pCMV6-ENTRY BubR1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BubR1 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Flow-Cytometry-NBP1-47680-img0011.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0003.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0004.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0005.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded colon tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0006.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded endometrium tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0007.jpg)
![Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded pancreas tissue using anti-BubR1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47680-img0008.jpg)
![Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.Lane 1: BUB1B transfected lysate(119.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0012.jpg "Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.Lane 1: BUB1B transfected lysate(119.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Immunohistochemistry-Paraffin-H00000701-M02-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi ( Cat # H00000701-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody (M02) clone 2G5 (Cat # H00000701-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0009.jpg)
![Western Blot: BubR1 Antibody (2G5) [H00000701-M02] - BUB1B monoclonal antibody (M02), clone 2G5 Analysis of BUB1B expression in Hela S3 NE.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Western-Blot-H00000701-M02-img0011.jpg)
![Proximity Ligation Assay: BubR1 Antibody (2G5) [H00000701-M02] - Analysis of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Proximity-Ligation-Assay-H00000701-M02-img0006.jpg)
![Sandwich ELISA: BubR1 Antibody (2G5) [H00000701-M02] - Detection limit for recombinant GST tagged BUB1B is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BubR1-Antibody-2G5-Sandwich-ELISA-H00000701-M02-img0010.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Mouse and Rat BubR1 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BubR1 Antibody [NB100-579] - Detection of Human BubR1 on HeLa whole cell lysate using NB100-579. BubR1 was also immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0002.jpg)
![Western Blot: BubR1 Antibody [NB100-579] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody NB100-579 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NB100-579-img0001.jpg)
![Western Blot: BubR1 Antibody (S05-3G7) [NBP3-19840] - Western blot detection of BubR1 in CHO-K1, Ramos cell lysates using NBP3-19840 (1:1000 diluted). Predicted band size: 120kDa. Observed band size: 120kDa.](http://images.novusbio.com/fullsize/BubR1-Antibody-S05-3G7-Western-Blot-NBP3-19840-img0002.jpg "Western Blot: BubR1 Antibody (S05-3G7) [NBP3-19840] - Western blot detection of BubR1 in CHO-K1, Ramos cell lysates using NBP3-19840 (1:1000 diluted). Predicted band size: 120kDa. Observed band size: 120kDa.")
![Immunohistochemistry-Paraffin: BubR1 Antibody (S05-3G7) [NBP3-19840] - Immunohistochemistry of BubR1 in paraffin-embedded Human lung cancer tissue using NBP3-19840 at dilution 1/50](http://images.novusbio.com/fullsize/BubR1-Antibody-S05-3G7-Immunohistochemistry-Paraffin-NBP3-19840-img0001.jpg)
![Western Blot: BubR1 Antibody [NBP3-18818] - Immunoprecipitation/ Western blot analysis on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry](http://images.novusbio.com/fullsize/BubR1-Antibody-Western-Blot-NBP3-18818-img0001.jpg "Western Blot: BubR1 Antibody [NBP3-18818] - Immunoprecipitation/ Western blot analysis on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry")
![Western Blot: BubR1 Antibody (7H4) [NBP3-15207] - Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate. All lanes: BubR1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120, 106, 122 KDa. Observed band size: 125 KDa.](http://images.novusbio.com/fullsize/BubR1-Antibody-7H4-Western-Blot-NBP3-15207-img0002.jpg "Western Blot: BubR1 Antibody (7H4) [NBP3-15207] - Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate. All lanes: BubR1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120, 106, 122 KDa. Observed band size: 125 KDa.")
![Flow Cytometry: BubR1 Antibody (7H4) [NBP3-15207] - Overlay histogram showing Hela cells stained with BubR1 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/BubR1-Antibody-7H4-Flow-Cytometry-NBP3-15207-img0001.jpg)
![SDS-Page: Recombinant Human BubR1 Protein [H00000701-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00000701-P01-1.jpg "SDS-Page: Recombinant Human BubR1 Protein [H00000701-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: BubR1 Overexpression Lysate (Adult Normal) [NBL1-08057] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BubR1.](http://images.novusbio.com/fullsize/BubR1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08057-img0002.jpg "Western Blot: BubR1 Overexpression Lysate (Adult Normal) [NBL1-08057] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BubR1.")