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BRD4 Antibody - Azide and BSA Free

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Western Blot: BRD4 Antibody [NBP3-15953] - Western blot analysis of extracts of HeLa cells, using BRD4 antibody (NBP3-15953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: BRD4 Antibody [NBP3-15953] - Western blot analysis of extracts of HeLa cells, using BRD4 antibody (NBP3-15953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP3-15953] - Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] BRD4 Rabbit pAb (NBP3-15953) at dilution of 1:100 (40x lens).Perform ...read more
Genetic Strategies: Knockout Validated: BRD4 Antibody [NBP3-15953] - Western blot analysis of extracts from normal (control) and BRD4 Rabbit pAb knockout (KO) HeLa cells, using BRD4 Rabbit pAb antibody ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Validated by:
     

Genetic Strategies

    

Order Details

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Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-15953-20ul
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BRD4 Antibody - Azide and BSA Free Summary

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 600-722 of human BRD4 (NP_055114.1). ESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETGPA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRD4
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Knockout Validated
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol, pH7.3
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for BRD4 Antibody - Azide and BSA Free

  • BRD4
  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1
  • HUNK1bromodomain-containing protein 4
  • HUNKI
  • MCAP
  • Protein HUNK1

Background

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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BRD4 Antibody -
Azide and BSA Free
NBP3-15953
BRD4 Antibody - Azide and BSA Free
Species: Hu, Rt
Applications: WB, KO

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BRD4