![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-18874-img0034.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BRD4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for BRD4 Antibody (NBP1-18874)Find related products by research area.
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Epigenetic Control of Autophagy By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studi... Read full blog post. |
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Understanding the relationship between NUT and BET proteins in NMC NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati... Read full blog post. |
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NUT - A Protein Coding Gene The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating... Read full blog post. |
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![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-18874-img0033.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB")
![Immunoprecipitation: BRD4 Antibody [NBP1-18874] - Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD4 antibody NBP1-18874 used for IP at 3 ug per reaction. BRD4 was also immunoprecipitated by a different rabbit anti-BRD4 antibody and rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (NBP2-76393). For blotting immunoprecipitated BRD4, NBP2-76393 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 1 seconds.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunoprecipitation-NBP1-18874-img0035.jpg "Immunoprecipitation: BRD4 Antibody [NBP1-18874] - Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD4 antibody NBP1-18874 used for IP at 3 ug per reaction. BRD4 was also immunoprecipitated by a different rabbit anti-BRD4 antibody and rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (NBP2-76393). For blotting immunoprecipitated BRD4, NBP2-76393 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 1 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")