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Bcl-9 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Analysis in human fallopian tube and pancreas tissues. Corresponding BCL9 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Bcl-9 Antibody [NBP2-30472] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Staining of human kidney shows weak nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP2-30472] - Staining of human pancreas shows strong nuclear positivity in Langerhans cells, exocrine pancreas was negative as expected

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Bcl-9 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: FTGAQSGGPQQNPGVLDGPQKKPEGPIQAMMAQSQSLGKGPGPRTDVGAPFGPQGHRDVPFSPDEMVPPSMNSQSGTIGPDHLDHMTPEQIAWLKLQQEFYEEKRRKQEQVVVQQCSLQD
Predicted Species
Mouse (93%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Bcl-9 Protein (NBP2-30472PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Bcl-9 Antibody

  • B-cell CLL/lymphoma 9 protein
  • B-cell CLL/lymphoma 9
  • B-cell lymphoma 9 protein
  • Bcl9
  • Bcl-9
  • BCL9L
  • LGS
  • MGC131591
  • Protein legless homolog

Background

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-30472

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Bioinformatics

Gene Symbol BCL9
Uniprot