| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB |
| Clone | 466023 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human Pygopus‑1 Ala2-Ala419 Accession # Q9Y3Y4 |
| Specificity | Detects human and mouse Pygopus-1 in direct ELISAs and Western blots. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PYGO1 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
Human Pygopus-1 (PYGO1, the name of a legless Australian lizard) is a 45 kDa, 419 amino acid (aa) transcriptional coactivator. It contains four domains: an N‑terminal NLS (aa 35‑41), followed by a Pro-rich, Asn-rich, and zinc-finger PHD-type domain (aa 340‑398). It is a nuclear protein that acts in concert with BCL-9 to retain beta -catenin in the nucleus during Wnt-signaling. In particular, following beta -catenin activation and translocation to the nucleus, beta -catenin first binds to BCL-9 in a phosphorylation-independent manner. Its continued presence in the nucleus depends upon BCL-9 binding to Pygopus. Once anchored, beta -catenin interacts with TCF to activate Wnt-responsive genes. Human Pygopus-1 shares 87% aa sequence identity with mouse Pygopus-1.
![Immunocytochemistry Pygopus-2 Antibody (539730) [Unconjugated]](https://images.novusbio.com/images2/Pygopus-2_MAB3616_Immunocytochemistry_10465.jpg)
![Western Blot Pygopus-2 Antibody (539730) [Unconjugated]](https://images.novusbio.com/images2/Pygopus-2_MAB3616_Western_Blot_10485.jpg)
![Simple Western Pygopus-2 Antibody (539730) [Unconjugated]](https://images.novusbio.com/images2/Pygopus2_MAB3616_Simple_Western_16406.jpg)
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot BCL9-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Bcl9-2_AF4967_Western_Blot_5591.jpg)
![Bioactivity FGF18 [Unconjugated]](https://images.novusbio.com/images2/FGF18_8988F18_1072.jpg)
![SDS-PAGE FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF-8_423-F8_332.jpg)
![Bioactivity FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF8_423F8_1438.jpg)
![Cell Culture FGF-8 [Unconjugated]](https://images.novusbio.com/images/423-f8_recombinant-human-mouse-fgf-8b-protein-cell-culture-1052024145159..jpg)
![Bioactivity FGF-20 [Unconjugated]](https://images.novusbio.com/images/protein/2547-fgcf_recombinant-human-fgf-20-protein-cf-bioactivity-181120209345.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")