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Pygopus-2 Antibody (539730) [Unconjugated]

 
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Pygopus-2 Monoclonal Antibody (Catalog # MAB3616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pygopus-2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse ...read more
Pygopus-2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Pygopus-2 Monoclonal Antibody (Catalog # MAB3616) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Pygopus‑2 at approximately 65 kDa (as indicated) using 5 µg/mL of ...read more
Pygopus‑2 was detected in immersion fixed paraffin-embedded sections of normal human ovary (left panel) and ovarian cancer tissue (right panel) using Mouse Anti-Human Pygopus‑2 Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
539730
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3616-SP
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Pygopus-2 Antibody (539730) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pygopus-2
Met160-Glu336
Accession # NP_612157
Specificity
Detects human Pygopus-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Pygopus-1 or recombinant mouse Pygopus-1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PYGO2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pygopus-2 Antibody (539730) [Unconjugated]

  • 1190004M21Rik
  • FLJ33226
  • PYGO2
  • pygopus 2
  • pygopus homolog 2 (Drosophila)
  • pygopus homolog 2
  • Pygopus2
  • Pygopus-2

Background

Human Pygopus-2 (pygopus; the name of a legless Australian lizard) is a 41 kDa, 406 amino acid (aa) transcriptional coactivator.  It contains three domains: an N‑terminal NLS (aa 41-47), followed by a proline-rich region and a zinc-finger PHD-type domain (aa 330-387).  A potential internal alternate start site may generate an isoform that deletes the first 37 aa.  Pygopus 2 is a nuclear protein that acts in concert with BCL-9 and TCF to retain beta -Catenin in the nucleus during Wnt-signaling.  It does so by forming a “chain-of-adaptors” with the above three proteins.  Over the range of aa used for immunization, human pygopus 2 is 95% identical to mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PYGO2
Uniprot