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beta-Catenin Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat ...read more
HeLa human cervical epithelial carcinoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. beta ‑Catenin/DNA complexes were immuno­precipitated using 5 μg ...read more
beta -Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) at 15 µg/mL ...read more
beta -Catenin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) at ...read more
beta -Catenin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 15 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat beta ‑Catenin Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1329, filled histogram) or control antibody ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Catenin at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ChIP, CyTOF-ready, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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beta-Catenin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human beta -Catenin
Ala2-Leu781
Accession # P35222
Specificity
Detects human, mouse and rat beta -Catenin in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTNNB1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF1329 in the following applications:

Publications
Read Publications using
AF1329 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-Catenin Antibody [Unconjugated]

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Background

beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Catenin Antibody (AF1329)(33)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Hamster, Transgenic Mouse.

We have publications tested in 12 applications: Bioassay, ChIP, ChIP-Seq, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot.


Filter By Application
Bioassay
(1)
ChIP
(1)
ChIP-Seq
(1)
Functional Assay
(1)
ICC
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(4)
Immunocytochemistry
(1)
Immunohistochemistry
(1)
Immunoprecipitation
(2)
Western Blot
(13)
All Applications
Filter By Species
Human
(6)
Mouse
(14)
Rat
(1)
Chicken
(1)
Hamster
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using AF1329 Applications Species
Lin, LL;Nayak, B;Osmulski, PA;Wang, E;Wang, CP;Valente, PT;Wang, CM;Tan, X;Santanam, N;Wang, TL;Gaczynska, ME;Kost, ER;Huang, TH;Kirma, NB; PAI-1 uncouples integrin-?1 from restrain by membrane-bound ?-catenin to promote collagen fibril remodeling in obesity-related neoplasms Cell reports 2024-07-23 [PMID: 39046873] (Immunocytochemistry, Functional Assay, Human) Immunocytochemistry, Functional Assay Human
Prochera, A;Muppirala, AN;Kuziel, GA;Soualhi, S;Shepherd, A;Sun, L;Issac, B;Rosenberg, HJ;Karim, F;Perez, K;Smith, KH;Archibald, TH;Rakoff-Nahoum, S;Hagen, SJ;Rao, M; Enteric glia regulate Paneth cell secretion and intestinal microbial ecology bioRxiv : the preprint server for biology 2024-04-19 [PMID: 38659931] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Cai, X;Zhao, Y;Yang, Y;Wu, X;Zhang, L;Ma, JA;Ji, J;Boström, KI;Yao, Y; GSK3 beta Inhibition Ameliorates Atherosclerotic Calcification International journal of molecular sciences 2023-07-19 [PMID: 37511396] (Western Blot, Mouse) Western Blot Mouse
KI Boström, X Qiao, Y Zhao, X Wu, L Zhang, JA Ma, J Ji, X Cai, Y Yao GSK3beta Inhibition Reduced Vascular Calcification in Ins2Akita/+ Mice International Journal of Molecular Sciences, 2023-03-22;24(6):. 3/22/2023 [PMID: 36983045]
Nicolas Schall, François Daubeuf, Claire Marsol, Patrick Gizzi, Nelly Frossard, Dominique Bonnet et al. A Selective Neutraligand for CXCL12/SDF-1 alpha With Beneficial Regulatory Functions in MRL/Lpr Lupus Prone Mice Frontiers in Pharmacology 10/21/2021 [PMID: 34744730]
N Doumpas, S Söderholm, S Narula, S Moreira, BW Doble, C Cantù, K Basler TCF/LEF regulation of the topologically associated domain ADI promotes mESCs to exit the pluripotent ground state Cell Reports, 2021-09-14;36(11):109705. 2021-09-14 [PMID: 34525377] (Western Blot, Mouse) Western Blot Mouse
J Yao, X Wu, X Qiao, D Zhang, L Zhang, JA Ma, X Cai, KI Boström, Y Yao Shifting osteogenesis in vascular calcification JCI Insight, 2021-05-24;0(0):. 2021-05-24 [PMID: 33848269] (ChIP, ChIP-Seq, Western Blot, Mouse) ChIP, ChIP-Seq, Western Blot Mouse
XF Liu, K Wang, HC Du LncRNA SNHG6 regulating Hedgehog signaling pathway and affecting the biological function of gallbladder carcinoma cells through targeting miR-26b-5p Eur Rev Med Pharmacol Sci, 2020-07-01;24(14):7598-7611. 2020-07-01 [PMID: 32744686] (Western Blot, Human) Western Blot Human
S Yamazaki, C Gerhold, K Yamamoto, Y Ueno, R Grosse, K Miyamoto, M Harata The Actin-Family Protein Arp4 Is a Novel Suppressor for the Formation and Functions of Nuclear F-Actin Cells, 2020-03-19;9(3):. 2020-03-19 [PMID: 32204557] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Fei Han, Prasad Konkalmatt, Chaitanya Mokashi, Megha Kumar, Yanrong Zhang, Allen Ko et al. Dopamine D2 receptor modulates Wnt expression and control of cell proliferation Scientific Reports 11/14/2019 [PMID: 31727925]
Show All 33 Publications.

Reviews for beta-Catenin Antibody (AF1329) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using AF1329:
Filter by Applications
IF
(1)
IP
(1)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence beta-Catenin AF1329
Enlarge
4
reviewed by:
Verified Customer
IF Rat 04/03/2015
View

Summary

ApplicationImmunofluorescence
Sample Testedkidney
SpeciesRat
LotHZT0914071
  4
reviewed by:
Verified Customer
IP Human 01/05/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 23474492
SpeciesHuman

Product General Protocols

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FAQs for beta-Catenin Antibody (AF1329) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
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4
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Verified Customer
04/03/2015
Application: IF
Species: Rat

 
Verified Customer
01/05/2015
Application: IP
Species: Human

Bioinformatics

Gene Symbol CTNNB1
Uniprot