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AMPK alpha 2 [p Thr172] Antibody (4F4)

Images

 
Western Blot: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Positive Western Blot detected in 293 whole cell lysate A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: AMPK alpha 2 [p Thr172] ...read more
Immunocytochemistry/ Immunofluorescence: AMPK alpha 2 [p Thr172] Antibody (4F4) [NBP3-26400] - Staining of A549 cells with AMPK alpha 2 [p Thr172] Antibody (4F4) at 1:100, counter-stained with DAPI. The cells were fixed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4F4
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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AMPK alpha 2 [p Thr172] Antibody (4F4) Summary

Additional Information
Recombinant monoclonal antibody expressed in HEK293F cells
Immunogen
A synthesized peptide derived from Human AMPK alpha 2 [p Thr172] [UniProt P54646]
Modification
p Thr172
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PRKAA2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for AMPK alpha 2 [p Thr172] Antibody (4F4)

  • 5'-AMP-activated protein kinase catalytic subunit alpha-2
  • AMPK alpha 2
  • AMPK subunit alpha-2
  • AMPK2
  • AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
  • AMPK-alpha-2 chain
  • EC 2.7.11
  • EC 2.7.11.1
  • PRKAA
  • PRKAA2
  • protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AMPK alpha 2 Antibody (NBP3-26400) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on AMPK alpha 2.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

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Bioinformatics

Gene Symbol PRKAA2