![Western Blot: PYK2/FAK2 Antibody [NBP3-12969] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody (NBP3-12969) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP3-12969-img0005.jpg "Western Blot: PYK2/FAK2 Antibody [NBP3-12969] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody (NBP3-12969) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PYK2/FAK2. The exact sequence is proprietary. |
| Predicted Species | Bovine (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PTK2B |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for PYK2/FAK2 Antibody (NBP3-12969)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PTK2B |
![Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PYK2/FAK2 stained by PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12969-img0006.jpg "Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PYK2/FAK2 stained by PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunohistochemistry-Paraffin-NBP3-12969-img0004.jpg "Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody (NBP3-12969) diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Western-Blot-NBP3-12969-img0001.jpg "Western Blot: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody (NBP3-12969) diluted at a dilution of 1:1000.")
![Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PYK2/FAK2 protein stained by PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12969-img0002.jpg "Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PYK2/FAK2 protein stained by PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunoprecipitation: PYK2/FAK2 Antibody [NBP3-12969] - Immunoprecipitation of PYK2/FAK2 protein from A431 whole cell extracts using 5 ug of PYK2/FAK2 antibody (NBP3-12969). Western blot analysis was performed using PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PYK2-FAK2-Antibody-Immunoprecipitation-NBP3-12969-img0003.jpg "Immunoprecipitation: PYK2/FAK2 Antibody [NBP3-12969] - Immunoprecipitation of PYK2/FAK2 protein from A431 whole cell extracts using 5 ug of PYK2/FAK2 antibody (NBP3-12969). Western blot analysis was performed using PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")