VGLUT1 Antibody (SR2075) Summary
Additional Information |
Recombinant Monoclonal Antibody |
Immunogen |
A synthesized peptide derived from human VGLUT1 (Uniprot #: Q9P2U7) |
Specificity |
Detects endogenous levels of total VGLUT1 |
Isotype |
IgG |
Clonality |
Monoclonal |
Host |
Rabbit |
Gene |
SLC17A7 |
Purity |
Affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Western Blot 1:500-1:2000
|
Theoretical MW |
61 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS, pH 7.4, 150mM NaCl, 50% glycerol. |
Preservative |
0.02% Sodium Azide |
Purity |
Affinity purified |
Alternate Names for VGLUT1 Antibody (SR2075)
Background
VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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