Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - The decrease in protein expressions of c-kit, Choline Acetyltransferase/ChAT, nNOS and Cx43 in the colon of elderly humans. Expressions of c-kit, ...read more
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Representative images of lumbar spinal cord sections at 130 days immunostained with Choline Acetyltransferase/ChAT. Scale ...read more
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brainstem showing specific labeling using the anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.
Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brain lysate showing specific immunolabeling of the ~70k ChAT.
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Immunostaining of anterior horn of lumbar spinal cord for Choline Acetyltransferase/ChAT (in green) and p62 (in red) at 130 ...read more
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - MATR3 loss in alpha -motor neurons & interneurons in the thoracic spinal cord of Matr3S85C/S85C mice. (A) Representative ...read more
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - MATR3 loss in alpha -motor neurons & interneurons in the cervical spinal cord of Matr3S85C/S85C mice. (A) Representative ...read more
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 21912682) Use in Immunohistochemistry-whole mount reported in scientific literature (PMID 24630395). ICC/IF reported in scientific literature (PMID 23095258). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 30261285).
Theoretical MW
70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP1-30052 in the following applications:
Alternate Names for Choline Acetyltransferase/ChAT Antibody
acetyl CoA:choline O-acetyltransferase
CHAT
CHOACTase
Choline acetylase
Choline Acetyltransferase
choline O-acetyltransferase
CMS1A
CMS1A2
EC 2.3.1
EC 2.3.1.6
Background
Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.; Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.; CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine.; Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Reviews for Choline Acetyltransferase/ChAT Antibody (NBP1-30052) (0)
There are no reviews for Choline Acetyltransferase/ChAT Antibody (NBP1-30052).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our Choline Acetyltransferase/ChAT Antibody and receive a gift card or discount.
![Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - The decrease in protein expressions of c-kit, Choline Acetyltransferase/ChAT, nNOS and Cx43 in the colon of elderly humans. Expressions of c-kit, ChAT, nNOS and Cx43 proteins in colonic muscle layers detached from youth and older adults were examined by western blotting (n=4 per group), and similar reduction was observed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30530917/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Choline Acetyltransferase-ChAT Antibody-Western Blot-NBP1-30052-img0006.jpg "Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - The decrease in protein expressions of c-kit, Choline Acetyltransferase/ChAT, nNOS and Cx43 in the colon of elderly humans. Expressions of c-kit, ChAT, nNOS and Cx43 proteins in colonic muscle layers detached from youth and older adults were examined by western blotting (n=4 per group), and similar reduction was observed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30530917/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Representative images of lumbar spinal cord sections at 130 days immunostained with Choline Acetyltransferase/ChAT. Scale bar, 200 um. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30052-img0005.jpg "Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Representative images of lumbar spinal cord sections at 130 days immunostained with Choline Acetyltransferase/ChAT. Scale bar, 200 um. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brainstem showing specific labeling using the anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NBP1-30052-img0003.jpg "Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brainstem showing specific labeling using the anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.")
![Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brain lysate showing specific immunolabeling of the ~70k ChAT.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Western-Blot-NBP1-30052-img0002.jpg "Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brain lysate showing specific immunolabeling of the ~70k ChAT.")
![Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Immunostaining of anterior horn of lumbar spinal cord for Choline Acetyltransferase/ChAT (in green) and p62 (in red) at 130 days. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Choline-Acetyltransferase-ChAT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30052-img0004.jpg "Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Immunostaining of anterior horn of lumbar spinal cord for Choline Acetyltransferase/ChAT (in green) and p62 (in red) at 130 days. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888), licensed under a CC-BY license.")
![Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - MATR3 loss in alpha -motor neurons & interneurons in the thoracic spinal cord of Matr3S85C/S85C mice. (A) Representative images of 10 & 30 weeks thoracic spinal cord staining for alpha -motor neurons (ChAT+, NeuN+, denoted by white arrows) & gamma -motor neurons (Chat+, NeuN−, denoted by yellow arrows). Scale bar for the spinal cord image denotes 500 μm & the zoomed-in image denotes 50 µm. Quantification of the percentage of motor neurons with reduced MATR3 staining in (B) alpha -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (C) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Quantification of the number of (D) alpha -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (E) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (F) Representative images of 10 & 30 weeks PVALB+ interneurons in the thoracic spinal cord. Interneurons with reduced MATR3 staining are denoted by a white asterisk. Scale bar denotes 50 µm. (G) Quantification of the percentage of PVALB+ interneurons with reduced MATR3 staining (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (H) Quantification of the number of PVALB+ interneurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Bar graph heights depict mean ± SEM, with each datapoint representing an animal. * p < 0.05, *** p < 0.001, **** p < 0.0001, ns = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35205163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30052_goat-polyclonal-choline-acetyltransferase-chat-antibody-310202416173537.jpg "Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - MATR3 loss in alpha -motor neurons & interneurons in the thoracic spinal cord of Matr3S85C/S85C mice. (A) Representative images of 10 & 30 weeks thoracic spinal cord staining for alpha -motor neurons (ChAT+, NeuN+, denoted by white arrows) & gamma -motor neurons (Chat+, NeuN−, denoted by yellow arrows). Scale bar for the spinal cord image denotes 500 μm & the zoomed-in image denotes 50 µm. Quantification of the percentage of motor neurons with reduced MATR3 staining in (B) alpha -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (C) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Quantification of the number of (D) alpha -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (E) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (F) Representative images of 10 & 30 weeks PVALB+ interneurons in the thoracic spinal cord. Interneurons with reduced MATR3 staining are denoted by a white asterisk. Scale bar denotes 50 µm. (G) Quantification of the percentage of PVALB+ interneurons with reduced MATR3 staining (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (H) Quantification of the number of PVALB+ interneurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Bar graph heights depict mean ± SEM, with each datapoint representing an animal. * p < 0.05, *** p < 0.001, **** p < 0.0001, ns = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35205163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - MATR3 loss in alpha -motor neurons & interneurons in the cervical spinal cord of Matr3S85C/S85C mice. (A) Representative images of 10 & 30 weeks cervical spinal cord staining for alpha -motor neurons (ChAT+, NeuN+, denoted by white arrows) & gamma -motor neurons (ChAT+, NeuN−, denoted by yellow arrows). Scale bar for the spinal cord image denotes 500 μm & the zoomed-in image denotes 50 µm. Quantification of the percentage of motor neurons with reduced MATR3 staining in (B) alpha -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (C) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Quantification of the number of (D) alpha -motor neurons (10 weeks: n = 6 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (E) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (F) Representative images of 10 & 30 weeks PVALB+ interneurons in the cervical spinal cord. Interneurons with reduced MATR3 staining are denoted by a white asterisk. Scale bar denotes 50 µm. (G) Quantification of the percentage of PVALB+ interneurons with reduced MATR3 staining (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (H) Quantification of the number of PVALB+ interneurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Bar graph heights depict mean ± SEM, with each datapoint representing an animal. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35205163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30052_goat-polyclonal-choline-acetyltransferase-chat-antibody-310202416165640.jpg "Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - MATR3 loss in alpha -motor neurons & interneurons in the cervical spinal cord of Matr3S85C/S85C mice. (A) Representative images of 10 & 30 weeks cervical spinal cord staining for alpha -motor neurons (ChAT+, NeuN+, denoted by white arrows) & gamma -motor neurons (ChAT+, NeuN−, denoted by yellow arrows). Scale bar for the spinal cord image denotes 500 μm & the zoomed-in image denotes 50 µm. Quantification of the percentage of motor neurons with reduced MATR3 staining in (B) alpha -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (C) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Quantification of the number of (D) alpha -motor neurons (10 weeks: n = 6 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (E) gamma -motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (F) Representative images of 10 & 30 weeks PVALB+ interneurons in the cervical spinal cord. Interneurons with reduced MATR3 staining are denoted by a white asterisk. Scale bar denotes 50 µm. (G) Quantification of the percentage of PVALB+ interneurons with reduced MATR3 staining (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (H) Quantification of the number of PVALB+ interneurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Bar graph heights depict mean ± SEM, with each datapoint representing an animal. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35205163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![SDS-PAGE beta-NGF [Unconjugated]](https://images.novusbio.com/images2/beta-NGF_256-GF_321.jpg)
![Bioactivity beta-NGF [Unconjugated]](https://images.novusbio.com/images2/beta-NGF_256-GF_322.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
![Immunohistochemistry NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]](https://images.novusbio.com/images2/NGF_R_AF1157_Immunohistochemistry_6685.jpg)
![Western Blot NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]](https://images.novusbio.com/images2/NGF_R_AF1157_Western_Blot_17941.jpg)
![Immunohistochemistry GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Immunohistochemistry_6730.jpg)
![Western Blot GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Western_Blot_19950.jpg)
![Simple Western GAD1/GAD67 Antibody [Unconjugated]](https://images.novusbio.com/images2/GAD1_AF2086_Simple_Western_20168.jpg)
![Western Blot Butyrylcholinesterase/BCHE Antibody [Unconjugated]](https://images.novusbio.com/images2/Butyrylcholinesterase_AF9024_Western_Blot_19121.jpg)
![Immunohistochemistry Butyrylcholinesterase/BCHE Antibody [Unconjugated]](https://images.novusbio.com/images2/Butyrylcholinesterase_AF9024_Immunohistochemistry_19158.jpg)
