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GAD2/GAD65 Antibody [Unconjugated]

 (4 Publications)
 (1 Review)
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GAD2/GAD65 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using Goat Anti-Human GAD2/GAD65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2247) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal roots. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
GAD2/GAD65 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using Goat Anti-Human GAD2/GAD65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2247) at 15 µg/mL overnight ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2247-SP
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GAD2/GAD65 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GAD2/GAD65
Ala1-Ala150
Accession # Q05329
Specificity
Detects human GAD2/GAD65 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human GAD1/GAD67 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GAD2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF2247 in the following applications:
Publications
Read Publications using
AF2247 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAD2/GAD65 Antibody [Unconjugated]

  • EC 4.1.1
  • EC 4.1.1.15
  • GAD2
  • GAD65
  • GAD-65
  • GAD65MGC161605
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
  • glutamate decarboxylase 2
  • Glutamate decarboxylase 65 kDa isoform
  • Glutamate decarboxylase-2 (pancreas)
  • MGC161607,65 kDa glutamic acid decarboxylase

Background

Glutamic acid decarboxylases (GAD), which catalyze the formation of gamma-aminobutyric acid from L-glutamic acid, are detectable in different isoforms encoded by distinct genes. GAD2, also known as GAD65, is expressed in the brain and pancreatic islets. GAD2 has been implicated as an autoantigen in the autoimmune disease Stiff Man Syndrome and in insulin-dependent diabetes mellitus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GAD2/GAD65
Antibody [Unconjugate
AF2247
GAD2/GAD65 Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC

Publications for GAD2/GAD65 Antibody (AF2247)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, ICC.


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Bioassay
(1)
ICC
(2)
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Human
(3)
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Review for GAD2/GAD65 Antibody (AF2247) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2247:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GAD2/GAD65 AF2247
Enlarge 		5
reviewed by:
Lydia Taverne
 IHC-P Human 12/16/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedAdult brain
SpeciesHuman
LotCFEN0111051

Comments

CommentsConcentration of 1:50 (4 ug/mL) GAD65 antibody on normal human brain tissue, with 488-labeled secondary antibody (green fluor). Antigen retrieval was with citrate pH 6 buffer

Product General Protocols

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Video Protocols

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FAQs for GAD2/GAD65 Antibody (AF2247) (0)

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Secondary Antibodies

 

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Calculators

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Recent Reviews

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5
1
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1
0
Immunohistochemistry-Paraffin GAD2/GAD65 AF2247
Enlarge
 Lydia Taverne
12/16/2019
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol GAD2
Uniprot