![Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Western-Blot-NBP1-20136-img0002.jpg "Western Blot: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated overnight at 4C.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, Flow, Func, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide Free |
| Immunogen | A synthetic peptide from mouse EAAT2/GLT1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC1A2 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Although not tested this antibody may work in IHC-Frozen. Use in flow was reported in scientific literature (PMID: 23793269). Use in In-vivo and in ICC/IF reported in scientific literature (PMID 25581361). Use in In-situ Hybridization reported in scientific literature (PMID: 26150391). |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from whole antisera |
| Preservative | No Preservative |
| Purity | Unpurified |
| Reconstitution Instructions | Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format. |
Secondary Antibodies |
Isotype Controls |
Research Areas for EAAT2/GLT1 Antibody (NBP1-20136)Find related products by research area.
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Deriving neural precursor cells from human induced pluripotent stem cells By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Human NPCs differentiate into mature neurons. Immunofluorescence staining of differentiated neurons derived from human dorsal NPCs (1323-2 line, day 35 after differentiation) for mature cortical neuronal markers expressed in the nucleus (BRN2, TBR1, NeuN) and cytoplasm (MAP2), glial markers (SOX9, GFAP, GLT1), and dorsal forebrain marker (FOXG1). Nuclei stained with DAPI, shown as an overlay over brightfield images. The merge is an overlay of the neuronal and glial markers. Scale bar 100 um. DAPI 4,6'-diamino-2-phenylindole Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-0812-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP1-20136-img0015.jpg "Immunocytochemistry/Immunofluorescence: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Human NPCs differentiate into mature neurons. Immunofluorescence staining of differentiated neurons derived from human dorsal NPCs (1323-2 line, day 35 after differentiation) for mature cortical neuronal markers expressed in the nucleus (BRN2, TBR1, NeuN) and cytoplasm (MAP2), glial markers (SOX9, GFAP, GLT1), and dorsal forebrain marker (FOXG1). Nuclei stained with DAPI, shown as an overlay over brightfield images. The merge is an overlay of the neuronal and glial markers. Scale bar 100 um. DAPI 4,6'-diamino-2-phenylindole Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-0812-6) licensed under a CC-BY license.")
![Immunohistochemistry: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Hippocampal GLT-1 and NR1 expression 3 days after SE with MyD88 inhibition. Sections from the hippocampi of mice in the CP group (A1-A3) and MIP group (B1-B3) 3 days after SE with GLT-1 immunoreactivity in astrocytes and neuronal processes. (A4, B4) Higher magnification of the boxes in (A3) and (B3). Scale bars: (A1-A3, B1-B3) 100 um; (A4, B4) 50 um. SE = status epilepticus, DG=dentate gyrus, hippocampal regions CA3 and CA1, CP = control peptide, MIP = MyD88 inhibitory peptide. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30112701/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EAAT2-GLT1 Antibody - Azide Free-Immunohistochemistry-NBP1-20136-img0016.jpg "Immunohistochemistry: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Hippocampal GLT-1 and NR1 expression 3 days after SE with MyD88 inhibition. Sections from the hippocampi of mice in the CP group (A1-A3) and MIP group (B1-B3) 3 days after SE with GLT-1 immunoreactivity in astrocytes and neuronal processes. (A4, B4) Higher magnification of the boxes in (A3) and (B3). Scale bars: (A1-A3, B1-B3) 100 um; (A4, B4) 50 um. SE = status epilepticus, DG=dentate gyrus, hippocampal regions CA3 and CA1, CP = control peptide, MIP = MyD88 inhibitory peptide. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30112701/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0003.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0004.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0005.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0006.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0007.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0008.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0009.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0010.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Mouse brain (hippocampus). The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0011.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0012.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0013.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.")
![Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/EAAT2-GLT1-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NBP1-20136-img0014.jpg "Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody - Azide Free [NBP1-20136] - Sections of mouse olfactory bulbs. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")