EN-RAGE/S100A12 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of EF-hand calcium-binding proteins. Like other S100 proteins, S100A12 is small and generally acidic (1-3). EN-RAGE forms noncovalent homodimers in the absence of divalent cations (4-6); calcium and zinc promote the formation of higher order assemblies including tetramers and hexamers (4, 7, 8). The ability of S100A12 to chelate zinc enables it to inhibit the zinc-dependent metalloproteases MMP-2, -3, and -9 (8). S100A12 also forms heterodimers with S100A9 and binds to RAGE (Receptor for Advanced Glycation End-products), Annexin V, and several cytosolic enzymes involved in energy metabolism (9, 10). The hexameric form of EN-RAGE in particular binds RAGE with high affinity (7). EN-RAGE induces a variety of inflammatory responses including the in vivo recruitment of neutrophils, monocytes, and mast cells and the activation of mast cells and vascular endothelial cells (9, 11-14). EN-RAGE is found at elevated levels under inflammatory conditions such as asthma, gout, rheumatoid arthritis synovial fluid, and atherosclerosis (8, 12, 14). S100A12 also promotes neurite outgrowth in isolated hippocampal neurons (15). An ortholog of S100A12 has not been identified in rodents, but the human protein is functional in mice and rats (11-16).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Bioassay, IHC-P, Western Blot.

Showing Publications 1 - 7 of 7.

Publications using AF1052	Applications	Species
Pierneef L, van Hooij A, de Jong D et al. Host biomarker-based quantitative rapid tests for detection and treatment monitoring of tuberculosis and COVID-19 iScience 12/26/2022 [PMID: 36590898]
Anouk van Hooij, Susan van den Eeden, Renate Richardus, Elisa Tjon Kon Fat, Louis Wilson, Kees L.M.C. Franken et al. Application of new host biomarker profiles in quantitative point-of-care tests facilitates leprosy diagnosis in the field EBioMedicine 9/1/2019 [PMID: 31422044]
Christoph Tondera, Markus Laube, Jens Pietzsch Insights into binding of S100 proteins to scavenger receptors: class B scavenger receptor CD36 binds S100A12 with high affinity Amino Acids 1/1/2017 [PMID: 27734162]
Cibin T Raghavan, Ram H Nagaraj AGE-RAGE interaction in the TGF?2-mediated epithelial to mesenchymal transition of human lens epithelial cells. Glycoconjugate Journal, 2016-06-04;0(0):1573-4986. 6/4/2016 [PMID: 27263094]
Shimamoto , Seiko, Kubota , Yasuo, Yamaguchi , Fuminori, Tokumitsu , Hiroshi, Kobayashi , Ryoji Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein). J Biol Chem, 2013-01-23;288(10):7158-68. 2013-01-23 [PMID: 23344957] (Bioassay, Human)	Bioassay	Human
S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis. Clin. Cancer Res., 2012-06-20;18(16):4356-64. 2012-06-20 [PMID: 22718861] (IHC-P, Human)	IHC-P	Human
Sharma I, Dhawan V, Mahajan N, Saha SC, Dhaliwal LK In vitro effects of atorvastatin on lipopolysaccharide-induced gene expression in endometriotic stromal cells. Fertil. Steril., 2009-11-26;94(5):1639. 2009-11-26 [PMID: 19944411] (Western Blot, Human)	Western Blot	Human

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	WB	Human	01/05/2015	View Summary Application Western Blot Sample Tested See PMID 19944411 Species Human
	4 reviewed by: Verified Customer	IHC-P	Human	01/05/2015	View Summary Application Immunohistochemistry-Paraffin Sample Tested See PMID 22609404 Species Human
	4 reviewed by: Verified Customer	IHC-P	Other	01/05/2015	View Summary Application Immunohistochemistry-Paraffin Sample Tested See PMID 22609404 Species Other

Array AF1052

• EN-RAGE/S100A12 Antibodies
• EN-RAGE/S100A12 ELISA Kits
• EN-RAGE/S100A12 Lysate
• EN-RAGE/S100A12 Protein
• EN-RAGE/S100A12 ELISA
• EN-RAGE/S100A12 Proteins and Enzyme