![Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/mL) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Intracellular-NB100-1869-img0011.jpg "Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Intracellular staining of HEK293 cells (1 x 10^6 cells/mL) with SLC1A3 antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide made to a C-terminal portion of the rat SLC1A3 protein (between residues 500-542) [Uniprot: P24942] |
| Localization | Membrane and ER |
| Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC1A3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 09/08/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869)Find related products by research area.
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![Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of SLC1A3 in rat cerebellum sections](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-NB100-1869-img0005.jpg "Immunohistochemistry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of SLC1A3 in rat cerebellum sections")
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 in 1. Human brain 2. Mouse brain and 3. Rat brain whole cell lysates.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Western-Blot-NB100-1869-img0014.jpg "Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 in 1. Human brain 2. Mouse brain and 3. Rat brain whole cell lysates.")
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - SLC1A3 antibody was tested at 1:250 in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1869-img0008.jpg "Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - SLC1A3 antibody was tested at 1:250 in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.")
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Human astrocytes express MHC-II in brain tissue from PD patients and healthy controls. The aSYN pathology was confirmed in the PD cases by immunostaining of aSYN and phosphorylated aSYN (p-aSYN). Astrocytes labeled with EAAT1/GLAST-1/SLC1A3 are expressing high levels of MHC-II, as well as Iba1-labeled microglia. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-01776-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1869-img0025.jpg "Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Human astrocytes express MHC-II in brain tissue from PD patients and healthy controls. The aSYN pathology was confirmed in the PD cases by immunostaining of aSYN and phosphorylated aSYN (p-aSYN). Astrocytes labeled with EAAT1/GLAST-1/SLC1A3 are expressing high levels of MHC-II, as well as Iba1-labeled microglia. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-01776-7), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 on mouse brain.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunohistochemistry-Paraffin-NB100-1869-img0006.jpg "Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Analysis of SLC1A3 on mouse brain.")
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - An intracellular stain was performed on rat FR cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody---BSA-Free-Flow-Cytometry-NB100-1869-img0026.jpg "Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - An intracellular stain was performed on rat FR cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).")
![Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Human astrocytes express MHC-II in brain tissue from PD patients and healthy controls. The aSYN pathology was confirmed in the PD cases by immunostaining of aSYN and phosphorylated aSYN (p-aSYN). Astrocytes labeled with EAAT1/GLAST-1/SLC1A3 are expressing high levels of MHC-II, as well as Iba1-labeled microglia. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-01776-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1869-img0024.jpg "Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Human astrocytes express MHC-II in brain tissue from PD patients and healthy controls. The aSYN pathology was confirmed in the PD cases by immunostaining of aSYN and phosphorylated aSYN (p-aSYN). Astrocytes labeled with EAAT1/GLAST-1/SLC1A3 are expressing high levels of MHC-II, as well as Iba1-labeled microglia. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-020-01776-7), licensed under a CC-BY license.")
![Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of HEK293 cells (1 x 10^6 cells/mL) with AF488 conjugated EAAT-1 antibody (orange) stained at a dilution of 1:500. Shown with rIgG (AF488) isotype control (blue).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Intracellular-NB100-1869-img0012.jpg "Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - Staining of HEK293 cells (1 x 10^6 cells/mL) with AF488 conjugated EAAT-1 antibody (orange) stained at a dilution of 1:500. Shown with rIgG (AF488) isotype control (blue).")
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 antibody NB100-1869PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0019.jpg "Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 antibody NB100-1869PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0020.jpg "Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0021.jpg "Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on U937 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).")
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Caco-2 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0022.jpg "Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Caco-2 cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).")
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Neuro2a cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/EAAT1-GLAST-1-SLC1A3-Antibody-Flow-Cytometry-NB100-1869-img0023.jpg "Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [NB100-1869] - An intracellular stain was performed on Neuro2a cells with SLC1A3 Antibody NB100-1869 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).")
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Damage response is restricted to MG. (A) RT-PCR analysis for Slc1a3, the gene encoding GLAST, at the indicated times after injury. (B) RT-PCR for MG & a photoreceptor-specific marker (Nrl) in GLAST-positive & negative fractions, after MACS in intact retinas. (C–F) qPCR quantification of Oct4, Nanog, Lin28, & Dnmt3b expression levels at the indicated time after injury in MACS GLAST-positive & negative fraction; C, intact retina as control (Student's t-test; ***p < 0.001; **p < 0.01; *p < 0.05). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnins.2016.00523/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415304223.jpg "Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Damage response is restricted to MG. (A) RT-PCR analysis for Slc1a3, the gene encoding GLAST, at the indicated times after injury. (B) RT-PCR for MG & a photoreceptor-specific marker (Nrl) in GLAST-positive & negative fractions, after MACS in intact retinas. (C–F) qPCR quantification of Oct4, Nanog, Lin28, & Dnmt3b expression levels at the indicated time after injury in MACS GLAST-positive & negative fraction; C, intact retina as control (Student's t-test; ***p < 0.001; **p < 0.01; *p < 0.05). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnins.2016.00523/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Damage response is restricted to MG. (A) RT-PCR analysis for Slc1a3, the gene encoding GLAST, at the indicated times after injury. (B) RT-PCR for MG & a photoreceptor-specific marker (Nrl) in GLAST-positive & negative fractions, after MACS in intact retinas. (C–F) qPCR quantification of Oct4, Nanog, Lin28, & Dnmt3b expression levels at the indicated time after injury in MACS GLAST-positive & negative fraction; C, intact retina as control (Student's t-test; ***p < 0.001; **p < 0.01; *p < 0.05). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnins.2016.00523/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415382418.jpg "Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Damage response is restricted to MG. (A) RT-PCR analysis for Slc1a3, the gene encoding GLAST, at the indicated times after injury. (B) RT-PCR for MG & a photoreceptor-specific marker (Nrl) in GLAST-positive & negative fractions, after MACS in intact retinas. (C–F) qPCR quantification of Oct4, Nanog, Lin28, & Dnmt3b expression levels at the indicated time after injury in MACS GLAST-positive & negative fraction; C, intact retina as control (Student's t-test; ***p < 0.001; **p < 0.01; *p < 0.05). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnins.2016.00523/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Simple Western: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Protein changes in ONHAs corroborate bioenergetics data. (A) Glucose transporter-1 protein levels in Stretched ONH astrocytes are significantly higher than Control (*p = 0.0225, n = 7 Control, n = 8 Stretch). Retinal lysate from a 2 month-old mouse was used as a positive control for each protein analyzed, while negative control was the signal obtained when no primary antibody was included in the capillary. (B) Lactate dehydrogenase-A, the astrocyte-specific isoform of the enzyme that catalyzes the interconversion of pyruvate & lactate, has equivalent protein levels in Control & Stretch ONH astrocytes. (C) Glucose-6-phosphate dehydrogenase, the enzyme that shunts glucose into the pentose phosphate pathway, is no different in Control & Stretch ONH astrocytes. (D) Glutamine synthetase, the enzyme that synthesizes glutamine from glutamate, is no different in Control & Stretch ONH astrocytes. (E) The monomeric form of glutamate-aspartate transporter (GLAST) has significantly higher protein levels in the Stretch as compared to the Control ONH astrocytes (p = 0.020; n = 4 Control, n = 5 Stretch). (F) GLAST dimer protein levels are no different in Control & Stretch ONH astrocytes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35992925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-31020241553387.jpg "Simple Western: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Protein changes in ONHAs corroborate bioenergetics data. (A) Glucose transporter-1 protein levels in Stretched ONH astrocytes are significantly higher than Control (*p = 0.0225, n = 7 Control, n = 8 Stretch). Retinal lysate from a 2 month-old mouse was used as a positive control for each protein analyzed, while negative control was the signal obtained when no primary antibody was included in the capillary. (B) Lactate dehydrogenase-A, the astrocyte-specific isoform of the enzyme that catalyzes the interconversion of pyruvate & lactate, has equivalent protein levels in Control & Stretch ONH astrocytes. (C) Glucose-6-phosphate dehydrogenase, the enzyme that shunts glucose into the pentose phosphate pathway, is no different in Control & Stretch ONH astrocytes. (D) Glutamine synthetase, the enzyme that synthesizes glutamine from glutamate, is no different in Control & Stretch ONH astrocytes. (E) The monomeric form of glutamate-aspartate transporter (GLAST) has significantly higher protein levels in the Stretch as compared to the Control ONH astrocytes (p = 0.020; n = 4 Control, n = 5 Stretch). (F) GLAST dimer protein levels are no different in Control & Stretch ONH astrocytes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35992925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Depletion of microglia promotes astrocyte responses following MPTP treatment. C57BL/6 mice received PLX3397 or vehicle for 21 d prior to saline or MPTP administration (intraperitoneal injection). Mice continued to receive PLX3397 or vehicle until the experiments ended. At d 7 after saline or MPTP injection, single cell suspensions were prepared from substantia nigra & striatal tissues. A) Flow cytometry plots show the gating strategy for astrocytes (GLAST+) & their expression of TNF-alpha , IL-6, iNOS, IP-10, CCL2, & CCL21. B, C) Bar graphs show the effects of microglial depletion on the expression of TNF-alpha , IL-6, iNOS, IP-10, CCL2, & CCL21 in astrocytes at d 7 after MPTP treatment. All data are presented as means ± sem; n = 9 mice/group. *P < 0.05, **P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29401614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415525277.jpg "Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Depletion of microglia promotes astrocyte responses following MPTP treatment. C57BL/6 mice received PLX3397 or vehicle for 21 d prior to saline or MPTP administration (intraperitoneal injection). Mice continued to receive PLX3397 or vehicle until the experiments ended. At d 7 after saline or MPTP injection, single cell suspensions were prepared from substantia nigra & striatal tissues. A) Flow cytometry plots show the gating strategy for astrocytes (GLAST+) & their expression of TNF-alpha , IL-6, iNOS, IP-10, CCL2, & CCL21. B, C) Bar graphs show the effects of microglial depletion on the expression of TNF-alpha , IL-6, iNOS, IP-10, CCL2, & CCL21 in astrocytes at d 7 after MPTP treatment. All data are presented as means ± sem; n = 9 mice/group. *P < 0.05, **P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29401614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Analysis of extracellular levels of GABA & Glutamate & membrane expression of their transporters. Extracellular levels of (A,C) GABA & glutamate were analyzed by microdialysis at 4 weeks. Membrane expression of (B) glutamate transporter GLAST & (D,E) GABA transporters GAT1 & GAT3 were analyzed using BS3 cross-linker. Values are expressed as percentage of control rats & are mean ± SEM of 12 rats per group. One-way ANOVA with Tukey’s test for GAT-1 (F(3,27) = 4.41, p < 0.05), GAT-3 (F(3,41) = 3.27, p < 0.05) & glutamate (F(3,36) = 3.00, p < 0.05) & Welch’s ANOVA with Dunnett’s test for GABA (W(3,19) = 3.67, p < 0.05) & GLAST (W(3,13) = 12.07, p < 0.001) were performed to compare all groups. Values significantly different from control rats are indicated by asterisks, from CCl4 rats by a, & from C-RIF rats by b. * p < 0.05, a p < 0.05, b p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1869_rabbit-polyclonal-eaat1-glast-1-slc1a3-antibody-310202415523910.jpg "Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody - BSA Free [NB100-1869] - Analysis of extracellular levels of GABA & Glutamate & membrane expression of their transporters. Extracellular levels of (A,C) GABA & glutamate were analyzed by microdialysis at 4 weeks. Membrane expression of (B) glutamate transporter GLAST & (D,E) GABA transporters GAT1 & GAT3 were analyzed using BS3 cross-linker. Values are expressed as percentage of control rats & are mean ± SEM of 12 rats per group. One-way ANOVA with Tukey’s test for GAT-1 (F(3,27) = 4.41, p < 0.05), GAT-3 (F(3,41) = 3.27, p < 0.05) & glutamate (F(3,36) = 3.00, p < 0.05) & Welch’s ANOVA with Dunnett’s test for GABA (W(3,19) = 3.67, p < 0.05) & GLAST (W(3,13) = 12.07, p < 0.001) were performed to compare all groups. Values significantly different from control rats are indicated by asterisks, from CCl4 rats by a, & from C-RIF rats by b. * p < 0.05, a p < 0.05, b p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")