Western Blot: USP9x Antibody (1C4) [H00008239-M01] - USP9x in mouse ovarian cancer cell lysate using anti-USP9x antibody. The primary antibody was used at a dilution of 1:500 and incubated for overnight at 4C in 5% milk ...read more
Immunocytochemistry/ Immunofluorescence: USP9x Antibody (1C4) [H00008239-M01] - Analysis of monoclonal antibody to USP9X on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: USP9x Antibody (1C4) [H00008239-M01] - USP9X expression in Hela NE ( Cat # L013V3 ).
ELISA: USP9x Antibody (1C4) [H00008239-M01] - Detection limit for recombinant GST tagged USP9X is approximately 0.1ng/ml as a capture antibody.
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
USP9X (NP_068706, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSSPDSSNENSPATPPDEQGQGDAPPQLEDEEPAFPHTDLAKLDDMINRPRWVVPVLP
Specificity
USP9X - ubiquitin specific peptidase 9, X-linked (fat facets-like, Drosophila)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
USP9X
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 20023629).
Reviewed Applications
Read 1 Review rated 4 using H00008239-M01 in the following applications:
ubiquitin specific peptidase 9, X-linked (fat facets-like, Drosophila)
ubiquitin specific peptidase 9, X-linked
ubiquitin thioesterase FAF-X
Ubiquitin thiolesterase FAF-X
ubiquitin-specific processing protease FAF-X
Ubiquitin-specific protease 9, X chromosome
Ubiquitin-specific-processing protease FAF-X
USP9
USP9x
X chromosome (fat facets-like Drosophila)
Background
This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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