![Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:1000.](http://images.novusbio.com/fullsize/SDHA-Antibody-GT20710-Western-Blot-NBP3-13522-img0009.jpg "Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, Bv, Ch, Xp, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | GT20710 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary. |
| Predicted Species | Chicken (97%), Xenopus (94%), Zebrafish (97%), Bovine (99%). Backed by our 100% Guarantee. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SDHA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 73 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SDHA Antibody (NBP3-13522)Find related products by research area.
|
|
SDHA - oxidative enzyme in the citric acid cycle Succinate dehydrogenase is an important tetrameric protein involved in the citric acid cycle. It is localized to the inner mitochondrial membrane of cells. Succinate dehydrogenase makes up Complex II of the electron transport chain (ETC) and is re... Read full blog post. |
|
SDHA - An essential Krebs cycle enzyme with role in cancer and metabolism Succinate dehydrogenase (SDH) is a highly conserved protein complex located on the inner mitochondrial membrane where it functions during the Krebs cycle by oxidizing succinate to fumarate (1). This reaction is also important for feeding electrons ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SDHA |
![Immunocytochemistry/Immunofluorescence: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHA protein stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/SDHA-Antibody-GT20710-Immunocytochemistry-Immunofluorescence-NBP3-13522-img0004.jpg "Immunocytochemistry/Immunofluorescence: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHA protein stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - Rat colon. SDHA stained by SDHA antibody [20710] (NBP3-13522) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SDHA-Antibody-GT20710-Immunohistochemistry-Paraffin-NBP3-13522-img0007.jpg "Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - Rat colon. SDHA stained by SDHA antibody [20710] (NBP3-13522) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000.](http://images.novusbio.com/fullsize/SDHA-Antibody-GT20710-Western-Blot-NBP3-13522-img0002.jpg "Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000.")
![Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000.](http://images.novusbio.com/fullsize/SDHA-Antibody-GT20710-Western-Blot-NBP3-13522-img0003.jpg "Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000.")
![Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SDHA-Antibody-GT20710-Western-Blot-NBP3-13522-img0008.jpg "Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - Mouse kidney. SDHA stained by SDHA antibody [20710] (NBP3-13522) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SDHA-Antibody-GT20710-Immunohistochemistry-Paraffin-NBP3-13522-img0005.jpg "Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - Mouse kidney. SDHA stained by SDHA antibody [20710] (NBP3-13522) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - Mouse liver. SDHA stained by SDHA antibody [20710] (NBP3-13522) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SDHA-Antibody-GT20710-Immunohistochemistry-Paraffin-NBP3-13522-img0006.jpg "Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - Mouse liver. SDHA stained by SDHA antibody [20710] (NBP3-13522) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13522_mouse-sdha-mab-gt20710-910202420104477.jpg "Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13522_mouse-sdha-mab-gt20710-9102024201461.jpg "Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/ Immunofluorescence: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.Green: SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:500.Red: MitoTracker® Red CMXRos, a mitochondria marker.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13522_mouse-sdha-mab-gt20710-91020242063335.jpg "Immunocytochemistry/ Immunofluorescence: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.Green: SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:500.Red: MitoTracker® Red CMXRos, a mitochondria marker.Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13522_mouse-sdha-mab-gt20710-910202420104494.jpg "Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13522_mouse-sdha-mab-gt20710-910202420115041.jpg "Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/DATA_Erythropoietin_MEP00_ELISA_970.jpg)
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/Erythropoietin_MEP00_ELISA_421.jpg)
![N/A PLGF [HRP]](https://images.novusbio.com/images2/PlGF_DPG00_ELISA_158.jpg)
![N/A PLGF [HRP]](https://images.novusbio.com/images2/DATA_PlGF_DPG00_ELISA_788.jpg)
![N/A PLGF [HRP]](https://images.novusbio.com/images2/DATA_PlGF_DPG00_ELISA_789.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")