Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0010.jpg "Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - Immunocytochemical analysis of USP9X in NTERA-2 cells](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48321-img0005.jpg)
![Immunohistochemistry: USP9x Antibody [NBP1-48321] - IHC staining of Usp9x in brain tissue.](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunohistochemistry-NBP1-48321-img0006.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in Caco-2 whole cell lysates.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0007.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in NIH/3T3 whole cell lysate.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - ICC/IC analysis of USP9x in mouse ES cell line 129. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48321-img0009.jpg)
![Western Blot: USP9x Antibody (1C4) [H00008239-M01] - USP9x in mouse ovarian cancer cell lysate using anti-USP9x antibody. The primary antibody was used at a dilution of 1:500 and incubated for overnight at 4C in 5% milk with PBST. Image from verified customer review.](http://images.novusbio.com/fullsize/USP9x-Antibody-1C4-Western-Blot-H00008239-M01-img0004.jpg "Western Blot: USP9x Antibody (1C4) [H00008239-M01] - USP9x in mouse ovarian cancer cell lysate using anti-USP9x antibody. The primary antibody was used at a dilution of 1:500 and incubated for overnight at 4C in 5% milk with PBST. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (1C4) [H00008239-M01] - Analysis of monoclonal antibody to USP9X on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/USP9x-Antibody-1C4-Immunocytochemistry-Immunofluorescence-H00008239-M01-img0006.jpg)
![Western Blot: USP9x Antibody (1C4) [H00008239-M01] - USP9X expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/USP9x-Antibody-1C4-Western-Blot-H00008239-M01-img0001.jpg)
![ELISA: USP9x Antibody (1C4) [H00008239-M01] - Detection limit for recombinant GST tagged USP9X is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-1C4-ELISA-H00008239-M01-img0008.jpg)
![Western Blot: USP9x Antibody (2G7) [NBP2-03824] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-USP9x monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Western-Blot-NBP2-03824-img0009.jpg "Western Blot: USP9x Antibody (2G7) [NBP2-03824] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-USP9x monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human prostate tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0008.jpg)
![Western Blot: USP9x Antibody (2G7) [NBP2-03824] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP9x (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP9x.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Western-Blot-NBP2-03824-img0001.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0002.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0003.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0004.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0005.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human Kidney tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0006.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human liver tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0007.jpg)
![Western Blot: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of USP9X on different lysates using anti-USP9X antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: A549Lane 3: 293 Lane 4: Mouse colon](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Western-Blot-NBP2-75704-img0007.jpg "Western Blot: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of USP9X on different lysates using anti-USP9X antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: A549Lane 3: 293 Lane 4: Mouse colon")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0004.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunohistochemistry-Paraffin-NBP2-75704-img0006.jpg)
![Flow Cytometry: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of 293T cells with USP9X antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Flow-Cytometry-NBP2-75704-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (JG35-11) [NBP2-75704] - Staining USP9X in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunocytochemistry-Immunofluorescence-NBP2-75704-img0003.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (JG35-11) [NBP2-75704] - Analysis of paraffin-embedded human tonsil tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.](http://images.novusbio.com/fullsize/USP9x-Antibody-JG35-11-Immunohistochemistry-Paraffin-NBP2-75704-img0005.jpg)
![Western Blot: USP9x Antibody (7T0S2) [NBP3-16884] - Western blot analysis of extracts of various cell lines, using USP9x Rabbit mAb (NBP3-16884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Western-Blot-NBP3-16884-img0004.jpg "Western Blot: USP9x Antibody (7T0S2) [NBP3-16884] - Western blot analysis of extracts of various cell lines, using USP9x Rabbit mAb (NBP3-16884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody (7T0S2) [NBP3-16884] - Immunofluorescence analysis of NIH-3T3 cells using USP9x Rabbit mAb (NBP3-16884) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Immunocytochemistry-Immunofluorescence-NBP3-16884-img0003.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (7T0S2) [NBP3-16884] - Immunohistochemistry of paraffin-embedded mouse testis using USP9x Rabbit mAb (NBP3-16884) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Immunohistochemistry-Paraffin-NBP3-16884-img0002.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (7T0S2) [NBP3-16884] - Immunohistochemistry of paraffin-embedded rat testis using USP9x Rabbit mAb (NBP3-16884) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/USP9x-Antibody-7T0S2-Immunohistochemistry-Paraffin-NBP3-16884-img0001.jpg)
![Western Blot: USP9x Antibody [NB100-61591] - Detection of Human and Mouse USP9X by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-USP9X antibody NB100-61591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. USP9X was also immunoprecipitated by rabbit anti-USP9X antibody NB100-61590, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NB100-61591-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: USP9x Antibody [NB100-61591] - Detection of Human and Mouse USP9X by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-USP9X antibody NB100-61591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. USP9X was also immunoprecipitated by rabbit anti-USP9X antibody NB100-61590, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).")