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UCP2 Antibody

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Western Blot: UCP2 Antibody [H00007351-D01P] - Analysis of UCP2 expression in transfected 293T cell line by UCP2 polyclonal antibody.Lane 1: UCP2 transfected lysate(33.20 KDa).Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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UCP2 Antibody Summary

Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Immunogen
UCP2 (NP_003346.2, 1 a.a. - 309 a.a.) full-length human protein. MVGFKATDVPPTATVKFLGAGTAACIADLITFPLDTAKVRLQIQGESQGPVRATASAQYRGVMGTILTMVRTEGPRSLYNGLVAGLQRQMSFASVRIGLYDSVKQFYTKGSEHASIGSRLLAGSTTGALAVAVAQPTDVVKVRFQAQARAGGGRRYQSTVNAYKTIAREEGFRGLWKGTSPNVARNAIVNCAELVTYDLIKDALLKANLMTDDLPCHFTSAFGAGFCTTVIASPVDVVKTRYMNSALGQYSSAGHCALTMLQKEGPRAFYKGFMPSFLRLGSWNVVMFVTYEQLKRALMAACTSREAPF
Specificity
Reacts with uncoupling protein 2 (mitochondrial, proton carrier).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UCP2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This antibody is reactive against transfected lysate in WB and as a detection antibody in ELISA.
Publications
Read Publication using
H00007351-D01P in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UCP2 Antibody

  • mitochondrial uncoupling protein 2
  • SLC25A8
  • SLC25A8BMIQ4
  • Solute carrier family 25 member 8
  • UCP 2
  • UCP2
  • UCPH
  • uncoupling protein 2 (mitochondrial, proton carrier)

Background

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UCP2 Antibody (H00007351-D01P)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for UCP2 Antibody (H00007351-D01P). (Showing 1 - 1 of 1 FAQ).

  1. Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.
    • Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.
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Bioinformatics

Gene Symbol UCP2
Entrez
Uniprot