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TRIP6 Antibody

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Western Blot: TRIP6 Antibody [NBP2-33667] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Western Blot: TRIP6 Antibody [NBP2-33667] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: TRIP6 Antibody [NBP2-33667] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: TRIP6 Antibody [NBP2-33667] -Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: TRIP6 Antibody [NBP2-33667] -Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: TRIP6 Antibody [NBP2-33667] -Staining of human testis shows strong nuclear positivity in Leydig cells.
Immunohistochemistry-Paraffin: TRIP6 Antibody [NBP2-33667] -Staining of human cervix, uterine shows strong cytoplasmic positivity in squamous epithelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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TRIP6 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RPGSLDAEIDLLSSTLAELNGGRGHASRRPDRQAYEPPPPPAYRTGSLKPNPASP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIP6
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TRIP6 Protein (NBP2-33667PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TRIP6 Antibody

  • MGC10556
  • MGC10558
  • MGC29959
  • MGC3837
  • MGC4423
  • OIP1OIP-1
  • Opa-interacting protein 1
  • thyroid hormone receptor interactor 6
  • thyroid receptor-interacting protein 6
  • TR-interacting protein 6
  • ZRP-1TRIP-6
  • zyxin related protein 1
  • Zyxin-related protein 1

Background

TRIP6 is a member of the zyxin family and encodes a protein with three LIM zinc-binding domains. This protein localizes to focal adhesion sites and along actin stress fibers. Recruitment of this protein to the plasma membrane occurs in a lysophosphatidic acid (LPA)-dependent manner and it regulates LPA-induced cell migration. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TRIP6
Uniprot