TopBP1 Antibody

Topoisomerases regulate topological states of DNA. DNA topoisomerase II-binding protein 1 (TopBP1) is a protein that interacts with the C-terminal region of topoisomerase II beta (Topo II beta). TopBP1 function has been found to be important to various aspects of cellular growth control such as DNA replication, the DNA damage checkpoint, and cell survival. TopBP1 interacts with several proteins through its BRCA1 C-terminal (BRCT) motifs. TopBP1 interacts and inhibits E2F1 transcriptional activity through an Rb-independent mechanism that involves recruitment of the SWI/SNF chromatin-remodeling complex proteins Brg1 and Brm. TopBP1 is also known as DNA topoisomerase 2-binding protein, DNA topoisomerase II beta-binding protein 1, TOPBP1, and KIAA0259.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.

Showing Publications 1 - 10 of 19. Show All 19 Publications. Collapse Publications.

Publications using NB100-217	Applications	Species
Dhoonmoon A, Schleicher EM, Clements KE et al. Genome-wide CRISPR synthetic lethality screen identifies a role for the ADP-ribosyltransferase PARP14 in DNA replication dynamics controlled by ATR Nucleic Acids Res. 2020-06-15 [PMID: 32542389] (Human)		Human
M Bosshard, R Aprigliano, C Gattiker, V Palibrk, E Markkanen, P Hoff Backe, S Pellegrino, FL Raymond, G Froyen, M Altmeyer, M Bjørås, GL Dianov, B van Loon Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability Sci Rep, 2017-11-08;7(1):15050. 2017-11-08 [PMID: 29118367]
Choe KN, Nicolae CM, Constantin D et al. HUWE1 interacts with PCNA to alleviate replication stress. EMBO Rep. 2016-05-04 [PMID: 27146073] (WB, Human)	WB	Human
Tomida J, Itaya A, Shigechi T et al. A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair. Nucleic Acids Res 2013-05-30 [PMID: 23723247] (WB, Human)	WB	Human
Ohlenschlager O, Kuhnert A, Schneider A et al. The N-terminus of the human RecQL4 helicase is a homeodomain-like DNA interaction motif Nucleic Acids Res 2012-06-22 [PMID: 22730300] (WB, IP, Human)	WB, IP	Human
Lee Y, Katyal S, Downing SM et al. Neurogenesis requires TopBP1 to prevent catastrophic replicative DNA damage in early progenitors. Nat Neurosci 2012-06-01 [PMID: 22522401]
Kuhnert A, Schmidt U, Monajembashi S et al. Proteomic identification of PSF and p54(nrb) as topBP1-interacting proteins J. Cell. Biochem 2012-01-01 [PMID: 22213094] (WB, Human)	WB	Human
Kumagai A, Shevchenko A, Shevchenko A et al. Direct regulation of Treslin by cyclin-dependent kinase is essential for the onset of DNA replication. J Cell Biol 2011-06-01 [PMID: 21646402]
Forrester NA, Sedgwick GG, Thomas A et al. Serotype-specific inactivation of the cellular DNA damage response during adenovirus infection. J Virol 2011-03-01 [PMID: 21159879]
Blackford AN, Patel RN, Forrester NA et al. Adenovirus 12 E4orf6 inhibits ATR activation by promoting TOPBP1 degradation. Proc Natl Acad Sci U S A 2010-07-01 [PMID: 20566845]
Guo R, Chen J, Zhu F et al. E2F1 localizes to sites of UV-induced DNA damage to enhance nucleotide excision repair. J Biol Chem 2010-06-01 [PMID: 20413589]
Sansam CL, Cruz NM, Danielian PS et al. A vertebrate gene, ticrr, is an essential checkpoint and replication regulator. Genes Dev 2010-01-15 [PMID: 20080954] (WB, Human)	WB	Human
Liu Y, Fang Y, Shao H et al. Interactions of human mismatch repair proteins MutSalpha and MutLalpha with proteins of the ATR-Chk1 pathway. J Biol Chem 2010-02-01 [PMID: 20029092]
Liu K, Bellam N, Lin HY et al. Regulation of p53 by TopBP1: a potential mechanism for p53 inactivation in cancer. Mol Cell Biol. 2009-05-01 [PMID: 19289498] Details: WB(human Saos-2 cells), Fig. 3D.
Xu X, Vaithiyalingam S, Glick GG et al. The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling. Mol Cell Biol 2008-12-01 [PMID: 18936170]
Hein J, Boichuk S, Wu J et al. Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding. J Virol 2009-01-01 [PMID: 18922873]
Delacroix S, Wagner JM, Kobayashi M et al. The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1. Genes Dev 2007-06-01 [PMID: 17575048]
Zhang D, Zaugg K, Mak TW et al. A role for the deubiquitinating enzyme USP28 in control of the DNA-damage response. Cell 2006-08-01 [PMID: 16901786]
Liu K, Luo Y, Lin FT et al. TopBP1 recruits Brg1/Brm to repress E2F1-induced apoptosis, a novel pRb-independent and E2F1-specific control for cell survival. Genes Dev 2004-03-01 [PMID: 15075294]
Show All 19 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Verified Customer	WB	Human	07/21/2010	View Summary Application Western Blot Sample Tested HeLa Cell Extract - Mitotic and Interphase, Sample Amount: Approximately 5x10^6 cell equivalents/lane Species Human

Array NB100-217

• TopBP1 Antibodies
• TopBP1 Lysate
• TopBP1 Proteins