TopBP1 Antibodies
We offer TOPBP1 Antibodies for use in common research applications: ELISA, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each TOPBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TOPBP1 Antibodies can be used in a variety of model species: Human. Use the list below to choose the TOPBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TOPBP1 polyclonal antibodies and browse our TOPBP1 monoclonal antibody catalog.
Alternate Names for TopBP1 Antibodies
anti-TopBP1 antibody, anti-DNA topoisomerase II-beta-binding protein 1 antibody, anti-DNA topoisomerase II-binding protein 1 antibody, anti-KIAA0259DNA topoisomerase 2-binding protein 1 antibody, anti-TOP2BP1 antibody, anti-topoisomerase (DNA) II binding protein 1 antibody
6 Results for "topbp1" in Primary Antibodies
![Western Blot: TopBP1 Antibody [NB100-217] - Detection of mouse TopBP1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TopBP1 antibody NB100-217 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TopBP1-Antibody-Western-Blot-NB100-217-img0027.jpg "Western Blot: TopBP1 Antibody [NB100-217] - Detection of mouse TopBP1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TopBP1 antibody NB100-217 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: TopBP1 Antibody [NB100-217] - Sample: Whole cell lysate (50 ug for WB) from HeLa cells.](http://images.novusbio.com/fullsize/TopBP1-Antibody-Western-Blot-NB100-217-img0022.jpg)
![Western Blot: TopBP1 Antibody [NB100-217] - WB analysis of TOPBP1 in HeLa cell extract mitotic and interphase. Image courtesy of product review submitted by Jennifer Flynn.](http://images.novusbio.com/fullsize/TopBP1-Antibody-Western-Blot-NB100-217-img0023.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TopBP1 Antibody [NB100-217] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOPBP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TopBP1-Antibody-Western-Blot-NB100-217-img0025.jpg)
![Western Blot: TopBP1 Antibody [NB100-217] - Detection of human TopBP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TopBP1 antibody NB100-217 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/TopBP1-Antibody-Western-Blot-NB100-217-img0026.jpg)
![Immunoprecipitation: TopBP1 Antibody [NB100-217] - Detection of human TopBP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TopBP1 antibody NB100-217 (lot NB100-217-2) used for IP at 3 ug per reaction. TopBP1 was also immunoprecipitated by a previous lot of this antibody (lot A300-111A-1). For blotting immunoprecipitated TopBP1, A300-111A was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/TopBP1-Antibody-Immunoprecipitation-NB100-217-img0028.jpg)
![Western Blot: TopBP1 Antibody (6D12) [H00011073-M04-100ug] - Detection against Immunogen (37.73 KDa) .](http://images.novusbio.com/fullsize/TopBP1-Antibody-6D12-Western-Blot-H00011073-M04-100ug-img0003.jpg "Western Blot: TopBP1 Antibody (6D12) [H00011073-M04-100ug] - Detection against Immunogen (37.73 KDa) .")
![Western Blot: TopBP1 Antibody (6D12) [H00011073-M04-100ug] - Monoclonal antibody (M04), clone 6D12. Western Blot analysis of TOPBP1 expression in Jurkat.](http://images.novusbio.com/fullsize/TopBP1-Antibody-6D12-Western-Blot-H00011073-M04-100ug-img0002.jpg)
![ELISA: TopBP1 Antibody (6D12) [H00011073-M04-100ug] - Detection limit for recombinant GST tagged TOPBP1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TopBP1-Antibody-6D12-ELISA-H00011073-M04-100ug-img0001.jpg)
![Western Blot: TopBP1 Antibody (PSH01-14) [NBP3-33036] - Western blot analysis of TopBP1 on different lysates with Rabbit anti-TopBP1 antibody (NBP3-33036) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HCT 116 cell lysate Lane 4: Jurkat cell lysate Lane 5: K-562 cell lysate Lane 6: HUVEC cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 171 kDa Observed band size: 171 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33036) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33036_rabbit-topbp1-mab-psh01-14-288202412493417.jpg "Western Blot: TopBP1 Antibody (PSH01-14) [NBP3-33036] - Western blot analysis of TopBP1 on different lysates with Rabbit anti-TopBP1 antibody (NBP3-33036) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HCT 116 cell lysate Lane 4: Jurkat cell lysate Lane 5: K-562 cell lysate Lane 6: HUVEC cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 171 kDa Observed band size: 171 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33036) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TopBP1 Antibody (PSH01-14) [NBP3-33036] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TopBP1 antibody (NBP3-33036) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33036) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33036_rabbit-topbp1-mab-psh01-14-298202414262687.jpg)
![Immunocytochemistry/Immunofluorescence: TopBP1 Antibody [NBP2-55484] - Staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize/TopBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55484-img0001.jpg "Immunocytochemistry/Immunofluorescence: TopBP1 Antibody [NBP2-55484] - Staining of human cell line U-2 OS shows localization to nucleus.")
![Immunocytochemistry/Immunofluorescence: TopBP1 Antibody [NBP2-55483] - Staining of human cell line A-431 shows localization to nucleus & nuclear bodies.](http://images.novusbio.com/fullsize/TopBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55483-img0001.jpg "Immunocytochemistry/Immunofluorescence: TopBP1 Antibody [NBP2-55483] - Staining of human cell line A-431 shows localization to nucleus & nuclear bodies.")