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Rad1 Antibody (1G2)

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Western Blot: Rad1 Antibody (1G2) [H00005810-M01J] - Western blot analysis of Rad1 over-expressed 293 cell line, cotransfected with Rad1 Validated Chimera RNAi [H00005810-R01V] (Lane 2) or non-transfected control (Lane 1). Blot probed with Rad1 monoclonal antibody (M01), clone 1G2 [H00005810-M01]. GAPDH ( 3
Western Blot: Rad1 Antibody (1G2) [H00005810-M01J] - Western blot analysis of Rad1 over-expressed 293 cell line, cotransfected with Rad1 Validated Chimera RNAi [H00005810-R01V] (Lane 2) or non-transfected control (Lane ...read more
Immunohistochemistry-Paraffin: Rad1 Antibody (1G2) [H00005810-M01J] - Immunoperoxidase of monoclonal antibody to Rad1 on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 3 ug/ml]
Western Blot: Rad1 Antibody (1G2) [H00005810-M01J] - Analysis of RAD1 expression in transfected 293T cell line by RAD1 monoclonal antibody (M01J), clone 1G2. Lane 1: RAD1 transfected lysate(32.43 KDa).Lane 2: ...read more
Western Blot: Rad1 Antibody (1G2) [H00005810-M01J] - Detection against Immunogen (35.53 KDa) .
Sandwich ELISA Capture: Rad1 Antibody (1G2) [H00005810-M01J] - Detection limit for recombinant GST tagged Rad1 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, ELISA, KD
Clone
1G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Rad1 Antibody (1G2) Summary

Immunogen
RAD1 (AAH06837, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLL
Specificity
RAD1 homolog (S. pombe)
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RAD1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Sandwich ELISA Capture
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
1X PBS pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rad1 Antibody (1G2)

  • cell cycle checkpoint protein Hrad1
  • checkpoint control protein HRAD1
  • DNA repair exonuclease rad1 homolog
  • DNA repair exonuclease REC1
  • EC 3.1.11.2
  • exonuclease homolog RAD1
  • HRAD1
  • RAD1 (S. pombe) homolog
  • RAD1 homolog (S. pombe)
  • Rad1-like DNA damage checkpoint protein
  • REC1cell cycle checkpoint protein RAD1

Background

This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RAD1
Entrez
OMIM
Uniprot