![Western Blot: RRAD Antibody [NBP2-27500] - Panel A: Staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 ug protein in RIPA buffer) with antibody at 0.5 ug/mL, and compared with an HA-specific antibody. Panel B: Staining of WT and KO lysates of Mouse Heart (100 ug protein in RIPA buffer) with antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA.](http://images.novusbio.com/fullsize/RRAD-Antibody-Western-Blot-NBP2-27500-img0003.jpg "Western Blot: RRAD Antibody [NBP2-27500] - Panel A: Staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10 ug protein in RIPA buffer) with antibody at 0.5 ug/mL, and compared with an HA-specific antibody. Panel B: Staining of WT and KO lysates of Mouse Heart (100 ug protein in RIPA buffer) with antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA.")
| Reactivity | Mu, Hu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, KO |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-HRRSMPVDERDLQ corresponding to internal region according to NP_004156.1. |
| Specificity | Reported variants represent identical protein: NP_004156.1, NP_001122322.1 |
| Predicted Species | Human (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | RRAD |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 37 kDa band observed in mouse heart lysates and no 37 kDa band in the KO lysates (calculated MW of 33.2 kDa band according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for other assays than WB. In transfected HEK293 transiently expressing mouse Rrad (HA tagged), a band of approx. 45 kDa is observed. No bands are observed in transfected HEK293 transiently expressing related mouse GTPases. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | RRAD |
| Entrez | |
| Uniprot |
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