TLR5 Antibody (85B152.5) - BSA Free

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 5 applications: FLOW, Flow Cytometry Control, Flow-CS, IHC-P, WB.

Showing Publications 1 - 10 of 28. Show All 28 Publications. Collapse Publications.

Publications using NBP1-97728	Applications	Species
Kumazoe M, Takamatsu K, Horie F et al. Methylated (-)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5 Sci Rep 2021-11-29 [PMID: 34845235] (FLOW, Mouse)	FLOW	Mouse
Hoang T, Jung J, Kim J All-Trans Retinoic Acid Enhances Bacterial Flagellin-Stimulated Proinflammatory Responses in Human Monocyte THP-1 Cells by Upregulating CD14 BioMed Research International 2019-12-27 [PMID: 31950055]
Mastorci K, Muraro E, Pasini E et al. Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma. PLoS ONE. 2016-04-29 [PMID: 27123851] (FLOW, Human) Details: This citation used the FITC version of this antibody.	FLOW	Human
Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine) Details: Used the PE form of this antibody.	FLOW	Canine
Kato K, Lillehoj EP, Kim KC. Pseudomonas aeruginosa stimulates tyrosine phosphorylation of and TLR5 association with the MUC1 cytoplasmic tail through EGFR activation. Inflamm. Res. 2016-03-01 [PMID: 26645913] (WB, Human)	WB	Human
Mishra A, Brown AL, Yao X et al. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase Nat Commun Nat Commun [PMID: 25692509] (FLOW, Mouse) Details: This publication used the PE conjugated form of this antibody (Cat# NBP2-24959).	FLOW	Mouse
Li Z, Younger K, Gartenhaus R et al. Inhibition of IRAK1/4 sensitizes T cell acute lymphoblastic leukemia to chemotherapies J Clin Invest 2015-03-02 [PMID: 25642772] (WB, Human) Details: This citation used the Biotin version of this antibody.	WB	Human
Bens M, Vimont S, Ben Mkaddem S et al. Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli Cell Microbiol 2014-04-29 [PMID: 24779433] (Flow Cytometry Control, Flow-CS, Mouse) Details: This citation used the PE version of this antibody.	Flow Cytometry Control, Flow-CS	Mouse
Sawitzki B, Brunstein C, Meisel C et al. Prevention of Graft-versus-Host Disease by Adoptive T Regulatory Therapy Is Associated with Active Repression of Peripheral Blood Toll-Like Receptor 5 mRNA Expression. Biol Blood Marrow Transplant [PMID: 24184334] (FLOW, Human) Details: Used the FITC form of this antibody.	FLOW	Human
Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013-04-08 [PMID: 23593278] (WB, Flow Cytometry Control) Details: Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL	WB, Flow Cytometry Control
Feng T, Cong Y, Alexander K, Elson CO. Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells. PLoS One. 2012-01-01 [PMID: 22545147] (Flow Cytometry Control, Mouse) Details: Antibodies cited: TLR5 (IMG-663A), Flow-intracellular (DC cells derived from C57BL/6 mice): Fig 1c, d: lamina propria (LP) from the intestine and splenic DCs. The cells were doubled stained with a CD11b mAb and the IMG-663A TLR5 mAb. The LP DCs but not th	Flow Cytometry Control	Mouse
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG et al. Toll-like receptor triggering in cord blood mesenchymal stem cells. J Cell Mol Med. 2009-09-01 [PMID: 20196781] (Flow-CS, Human) Details: flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.	Flow-CS	Human
Hafez M, Hayes K, Goldrick M et al. The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5, CD14, MyD88, and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells. Infect Immun. 2010-05-01 [PMID: 20145095] (Flow-CS) Details: IMGENEX products cited: 1. TLR4 (IMG- 5031A) Flow (Cell surface), Fig 3, Caco-2 cells. 2. TLR5 (IMG-663A) Flow (Cell surface), Fig 2, Fig 3, Caco-2 cells.	Flow-CS
He B, Xu W, Santini PA et al. Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL Immunity (IHC-P) Details: This publication used the FITC conjugated form of this antibody (Cat# NB200-571).	IHC-P
Rydberg C, Mansson A, Uddman R et al. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology. 2009-09-01 [PMID: 19740321] Details: TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315A), TLR3/CD283 (IMG-315D), TLR5 PE (IMG-663D), TLR5 (IMG-663). 1. IHC(paraffin): Human head and neck squamous (HNSCC) cell carcinomas showing keratinized cell carcinoma from the larynx stained with TLR2 (IMG-416A) and TLR5 antibodies (IMG-663A), Fig 1A, 1B, & 1C. 2. Flow (intracellular): TLR2 PE (IMG-416D), TLR3 PE (IMG-315D), and TLR5 PE (IMG-663D) antibodies were used in human bronchial epithelial (NL-20) and human pharyngeal squamous (Detroit-562) carcinoma cell line, Fig 2C.
Wu H, Wang H, Xiong W et al. Expression patterns and functions of toll-like receptors in mouse sertoli cells. Endocrinology. 2008-09-01 [PMID: 18499758] (Flow-CS, WB) Details: Antibodies cited: 1. TLR2 (IMG-410A): WB (sertoli cell lysates), Fig. 1D. 2. TLR5 (IMG-444A): WB (sertoli cell lysates), Fig. 1D. 3. TLR5 (IMG-664C): Flow (cell surface), mouse sertoli cells and macrophages, Figs. 1B,C,E. 4. TLR6 (IMG-527): WB (sertoli ce	Flow-CS, WB
Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008-07-15 [PMID: 18439687] Details: Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.
Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008-03-01 [PMID: 18292566] Details: Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
He B, Xu W, Santini PA et al. Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL Immunity 2007-06-01 [PMID: 17570691] (IHC-P) Details: This citation used the FITC version of this antibody.	IHC-P
Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440] Details: Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
Riccioli A, Starace D, Galli R et al. Sertoli cells initiate testicular innate immune responses through TLR activation J Immunol 2006-11-15 [PMID: 17082629] (Flow-CS) Details: This citation used the FITC version of this antibody.	Flow-CS
LeBouder E, Rey-Nores JE, Raby AC et al. Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk. J Immunol. 2006-03-15 [PMID: 16517743] (FLOW) Details: 1. TLR3 (IMG-315) [Flow (Cell Surface), Fig.3 (dendritic cells)]. 2. TLR5 (IMG-663) [Flow (Cell Surface), Fig.2 (dendritic cells)].	FLOW
Mansson A, Adner M, Cardell LO. Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis. Respir Res. 2006-02-27 [PMID: 16504163] (Flow Cytometry Control, Human) Details: Antibodies cited (human tonsils separated into cell subtypes): 1. TLR3 [IMG-315D (Flow-Intracellular), Figs 5 and 6]. 2. TLR5 [IMG-663A (Flow-Intracellular), Fig 6]. 3. TLR9 [IMG-305C (Flow-Intracellular), Fig 4.].	Flow Cytometry Control	Human
Crellin NK Garcia RV Hadisfar O et al. Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells. J Immunol. [PMID: 16339542] Details: Citation using the Azide and Endotoxin Free form of this antibody.
Francois S et al. Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad J Immunol [PMID: 15749901] Details: Used the FITC form of this antibody.
Chuang T, Ulevitch RJ. Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells. Biochim Biophys Acta. 2001-03-01 [PMID: 11267672]
Muzio M, Natoli G, Saccani S et al. The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6). J Exp Med. 1998-06-01 [PMID: 9625770]
Medzhitov R, Janeway CA Jr. Innate immunity: the virtues of a nonclonal system of recognition. Cell. 1997-10-01 [PMID: 9363937]
Show All 28 Publications. Collapse Publications.

Array NBP1-97728

• TLR5 Antibodies
• TLR5 ELISA Kits
• TLR5 Lysate
• TLR5 Protein
• TLR5 Proteins and Enzyme