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TGF-beta RII Antibody [Biotin]

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Myofibroblastic property of Replic cells. (a) mRNA expression levels of genes related to myofibroblasts in Replic cells cultured with MSCM. In addition to MEFs cultured with DMEM, injured and contralateral kidneys (n = 3 for each) of UUO-treated normal mice were compared to Replic cells with respect to mRNA expression. Twelve biologically independent samples for each cell line were analysed. (b) mRNA expression levels of genes for TGF beta superfamily receptors in Replic cells cultured with MSCM were compared to those in mouse kidneys (n = 3 for ISAM-REC kidneys and WT kidneys) and MEFs. Six biologically independent samples for each cell line were analysed. (c) Flow cytometry of TGF beta R2 expression on the cell surface of Replic cells and MEFs cultured with DMEM. Negative controls (NC) were stained only with APC-conjugated streptavidin. The mean fluorescent intensities are shown (red dotted lines). (d) mRNA expression levels of genes related to myofibroblasts in Replic cells cultured with DMEM or MSCM. Six biologically independent samples for each cell line were analysed. The average expression levels of injured kidneys (a), ISAM-REC kidneys (b), or Replic cells cultured with DMEM (d) were set at 1.0. Error bars are indicated standard errors, and arrows indicate undetectable levels (a,b). *p < 0.05 and **p < 0.01 by multiple comparisons using one-way ANOVA with Tukey-Kramer tests (a,b) or by two-tailed, unpaired Student’s t-tests (d). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31375751), licensed under a CC-BY license. Not internally tested by R&D Systems.
Myofibroblastic property of Replic cells. (a) mRNA expression levels of genes related to myofibroblasts in Replic cells cultured with MSCM. In addition to MEFs cultured with DMEM, injured and contralateral kidneys ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 700
FAB532N-100
Alexa Fluor 700
FAB532N-025
Allophycocyanin
FAB532A-100
Allophycocyanin
FAB532A-025
Fluorescein
FAB532F-100
Fluorescein
FAB532F-025
Phycoerythrin
FAB532P-100UG
Phycoerythrin
FAB532P-025UG
Unconjugated
AF532
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF532
Carrier Free
AF532
Carrier Free
FAB532F-100
Carrier Free
FAB532A-100
Carrier Free
FAB532N-100
Carrier Free
FAB532A-025
Carrier Free
FAB532F-025
Carrier Free
FAB532N-025
Carrier Free
AF532-SP
Carrier Free
FAB532P-100UG
Carrier Free
FAB532P-025UG
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TGF-beta RII Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TGF‑ beta  RII and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta  RII
Ile24-Asp184
Accession # Q62312
Specificity
Detects mouse TGF‑ beta  RII in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) TGF‑ beta  RII and less than 2% cross-reactivity with recombinant mouse TGF‑ beta  RI and rhTGF‑ beta  RIII is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFBR2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Readout System
Publications
Read Publications using
BAF532 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RII Antibody [Biotin]

  • AAT3
  • EC 2.7.11
  • EC 2.7.11.30
  • FAA3
  • HNPCC6
  • LDS1B
  • LDS2B
  • MFS2
  • RIIC
  • TAAD2
  • tbetaR-II
  • TGF-beta receptor type II
  • TGF-beta receptor type IIB
  • TGF-beta receptor type-2
  • TGF-beta RII
  • TGF-beta type II receptor
  • TGFbetaRII
  • TGFbeta-RII
  • TGFBR2
  • TGF-bRII
  • TGFR-2
  • transforming growth factor beta receptor type IIC
  • transforming growth factor, beta receptor II (70/80kDa) isoform 1
  • transforming growth factor, beta receptor II (70/80kDa) isoform 2
  • transforming growth factor, beta receptor II (70/80kDa)
  • transforming growth factor, beta receptor II (70-80kD)
  • Transforming growth factor-beta receptor type II

Background

Most cell types express three sizes of receptors for TGF-beta . These are designated Type I (53 kDa), Type II (70 - 85 kDa), and Type III (250 - 350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta . Evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors (1).

The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta .

  1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGF-beta RII Antibody (BAF532)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(5)
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Mouse
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Showing Publications 1 - 10 of 10.
Publications using BAF532 Applications Species
Shruti Mishra, Wei Liao, Yong Liu, Ming Yang, Chaoyu Ma, Haijing Wu et al. TGF-beta and Eomes control the homeostasis of CD8+ regulatory T cells Journal of Experimental Medicine 1/4/2021 [PMID: 32991667]
Shruti Mishra, Wei Liao, Yong Liu, Ming Yang, Chaoyu Ma, Haijing Wu et al. TGF-beta and Eomes control the homeostasis of CD8+ regulatory T cells Journal of Experimental Medicine 2021-01-04 [PMID: 32991667] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Koji Sato, Ikuo Hirano, Hiroki Sekine, Kenichiro Miyauchi, Taku Nakai, Koichiro Kato et al. An immortalized cell line derived from renal erythropoietin-producing (REP) cells demonstrates their potential to transform into myofibroblasts Scientific Reports 8/2/2019 [PMID: 31375751]
Victor S Cortez, Tyler K Ulland, Luisa Cervantes-Barragan, Jennifer K Bando, Michelle L Robinette, Qianli Wang et al. SMAD4 impedes the conversion of NK cells into ILC1-like cells by curtailing non-canonical TGF-beta signaling Nature Immunology 9/1/2017 [PMID: 28759002]
Anne L. Ruiz, Saidi M’Homa Soudja, Cyril Deceneux, Grégoire Lauvau, Julien C. Marie NK1.1+ CD8+ T Cells Escape TGF-beta control and Contribute to Early Microbial Pathogen-response Nature Communications 10/6/2014 [PMID: 25284210]
McCarron M, Marie J TGF-beta prevents T follicular helper cell accumulation and B cell autoreactivity. J Clin Invest, 2014-08-26;124(10):4375-86. 2014-08-26 [PMID: 25157822] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Ouyang W, Oh S, Ma Q, Bivona M, Zhu J, Li M TGF-beta cytokine signaling promotes CD8+ T cell development and low-affinity CD4+ T cell homeostasis by regulation of interleukin-7 receptor alpha expression. Immunity, 2013-08-08;39(2):335-46. 2013-08-08 [PMID: 23932572] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Lara Longobardi, Tieshi Li, Timothy J. Myers, Lynda O'Rear, Huseyin Ozkan, Ying Li et al. TGF-beta Type II Receptor/MCP-5 Axis: At the Crossroad between Joint and Growth Plate Development Developmental Cell 7/17/2012 [PMID: 22814601]
Radulovic K, Manta C, Rossini V, Holzmann K, Kestler HA, Wegenka UM, Nakayama T, Niess JH CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential. J. Immunol., 2012-01-16;188(4):2001-13. 2012-01-16 [PMID: 22250092] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Denton CP, Zheng B, Evans LA, Shi-wen X, Ong VH, Fisher I, Lazaridis K, Abraham DJ, Black CM, de Crombrugghe B Fibroblast-specific expression of a kinase-deficient type II transforming growth factor beta (TGFbeta) receptor leads to paradoxical activation of TGFbeta signaling pathways with fibrosis in transgenic mice. J. Biol. Chem., 2003-04-21;278(27):25109-19. 2003-04-21 [PMID: 12707256] (Flow Cytometry, Mouse) Flow Cytometry Mouse

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Bioinformatics

Gene Symbol TGFBR2
Uniprot