| Reactivity | MuSpecies Glossary |
| Applications | Flow |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Fluorescein |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 700 | FAB532N-100 | |||
| Alexa Fluor 700 | FAB532N-025 | |||
| Allophycocyanin | FAB532A-100 | |||
| Allophycocyanin | FAB532A-025 | |||
| Biotin | BAF532 | |||
| Fluorescein | FAB532F-025 | |||
| Phycoerythrin | FAB532P-100UG | |||
| Phycoerythrin | FAB532P-025UG | |||
| Unconjugated | AF532 | |||
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse TGF-beta RII and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF-beta RII Ile24-Asp184 Accession # Q62312 |
| Specificity | Detects mouse TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) TGF-beta RII is observed and less than 1% cross-reactivity with recombinant mouse TGF-beta RI and rhTGF-beta RIII is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TGFBR2 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Protect from light. Do not freeze.
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| Buffer | Supplied in a saline solution containing BSA and Sodium Azide. |
| Preservative | Sodium Azide |
Most cell types express three sizes of receptors for TGF-beta . These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta . Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.
The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta .
![TGF-beta RII Antibody [Fluorescein]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "TGF-beta RII Antibody [Fluorescein]")
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
IHC-Fr | Mouse | 12/09/2016 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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