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TGF-beta 3 Antibody [Unconjugated]

 (12 Publications)
 (1 Review)
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Recombinant Human TGF‑ beta 3 (Catalog # 243-B3) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of TGF‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA). The ND50 is typically 0.01-0.05 µg/mL.
Recombinant Human TGF‑ beta 3 (Catalog # 243-B3) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of ...read more
TGF‑ beta 3 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-TGF‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) at 5 µg/mL ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF243
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF-243-SP
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TGF-beta 3 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant chicken TGF‑ beta 3
Ala301-Ser412 (Tyr340Phe)
Accession # P10600
Specificity
Detects TGF-beta 3 in direct ELISAs. 
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFB3
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.01-0.05 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF-243-NA in the following applications:
Publications
Read Publications using
AF-243-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 3 Antibody [Unconjugated]

  • ARVD
  • ARVD1
  • FLJ16571
  • LDS5
  • RNHF
  • TGFB3
  • TGFbeta 3
  • TGF-beta 3
  • TGF-beta3
  • TGF-beta-3
  • transforming growth factor beta-3
  • transforming growth factor, beta 3

Background

TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8‑10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity (1). TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1988) EMBO J. 7:3737.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGF-beta 3 Antibody (AF-243-NA)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IHC, Neutralization.


Filter By Application
IHC
(1)
Neutralization
(4)
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Human
(3)
Mouse
(1)
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(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF-243-NA Applications Species
I Hanson, KE Pitman, U Altanerova, ? Altaner, E Malinen, NFJ Edin Low-Dose-Rate Radiation-Induced Secretion of TGF-beta3 Together with an Activator in Small Extracellular Vesicles Modifies Low-Dose Hyper-Radiosensitivity through ALK1 Binding International Journal of Molecular Sciences, 2022-07-24;23(15):. 2022-07-24 [PMID: 35897723] (Neutralization, Human) Neutralization Human
Jung Eun Baik, Hyeung Ju Park, Raghu P. Kataru, Ira L. Savetsky, Catherine L. Ly, Jinyeon Shin et al. TGF‐ beta 1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation Clinical and Translational Medicine 2022-06-02 [PMID: 35652284]
Yun Zhang, Hong-Mei Tang, Chun-Feng Liu, Xie-Fang Yuan, Xiao-Yun Wang, Ning Ma et al. TGF-beta 3 Induces Autophagic Activity by Increasing ROS Generation in a NOX4-Dependent Pathway Mediators of Inflammation 2019-12-31 [PMID: 32082074]
Chen X, Yang B, Tian J et al. Dental Follicle Stem Cells Ameliorate Lipopolysaccharide-Induced Inflammation by Secreting TGF-b3 and TSP-1 to Elicit Macrophage M2 Polarization Cell. Physiol. Biochem. 12/7/2018 [PMID: 30537736]
Nina Jeppesen Edin, Čestmír Altaner, Veronica Altanerova, Peter Ebbesen TGF-B3 Dependent Modification of Radiosensitivity in Reporter Cells Exposed to Serum from Whole-Body Low Dose-Rate Irradiated Mice Dose-Response 2015-12-18 [PMID: 26673923]
Maria J. Serrano, Jingpeng Liu, Kathy K.H. Svoboda, Ali Nawshad, M. Douglas Benson Ephrin reverse signaling mediates palatal fusion and epithelial-to-mesenchymal transition independently of tgf beta 3 Journal of Cellular Physiology 2018-04-11 [PMID: 25893671]
Kang I, Yoon D, Braun K, Wight T Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion. J Biol Chem, 2014-04-09;289(22):15393-404. 2014-04-09 [PMID: 24719328] (Neutralization, Rat) Neutralization Rat
Le M, Naridze R, Morrison J, Biggs L, Rhea L, Schutte B, Kaartinen V, Dunnwald M Transforming growth factor Beta 3 is required for excisional wound repair in vivo. PLoS ONE, 2012-10-26;7(10):e48040. 2012-10-26 [PMID: 23110169] (Neutralization, Mouse) Neutralization Mouse
Kathryn E LeMasters, Yotam Blech-Hermoni, Samantha J Stillwagon, Natalie A Vajda, Andrea N Ladd Loss of muscleblind-like 1 promotes invasive mesenchyme formation in endocardial cushions by stimulating autocrine TGF beta 3 BMC Developmental Biology 2012-12-01 [PMID: 22866814]
Natalie A. Vajda, Kyle R. Brimacombe, Kathryn E. LeMasters, Andrea N. Ladd Muscleblind-like 1 is a negative regulator of TGF-β-dependent epithelial–mesenchymal transition of atrioventricular canal endocardial cells Developmental Dynamics 2010-03-15 [PMID: 19890912]
Show All 12 Publications.

Review for TGF-beta 3 Antibody (AF-243-NA) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF-243-NA:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot TGF-beta 3 AF-243-NA
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 04/09/2017
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Summary

ApplicationWestern Blot
Sample TestedHuman MCF-7 cell lysate
SpeciesHuman
LotAAT051703A

Comments

CommentsExpression of TGF-beta 3 in breast cancer cell lysate using Goat anti-Human TGF-beta 3 antibody (#AF-243) at 1 ug/mL, followed by Donkey anti-Goat secondary antibody at 1:1,000 dilution. PVDF membrane blocked with 5% BSA-PBS.

FAQs for TGF-beta 3 Antibody (AF-243-NA) (0)

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Western Blot TGF-beta 3 AF-243-NA
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 Verified Customer
04/09/2017
Application: WB
Species: Human
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Bioinformatics

Gene Symbol TGFB3
Uniprot