I found the following products on your webpage: Aggrecan Neoepitope Antibody (NB100-74350), Aggrecan Neoepitope Antibody [HRP] (NB100-74350H), and Aggrecan Neoepitope Antibody [Biotin] (NB100-74350B). It is clear that both tagged antibodies are directed against the NITEGE neoepitope of aggrecan, but it is not clear for the non tagged antibody. Looking at the datasheets, those three antibodies look quite the same. Can you confirm that the non tagged antibody is also directed against the NITEGE neoepitope? The immunogen indicated in the datasheet is "A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400)"

Name: Aggrecan Neoepitope Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB100-74350
Price: 399 USD

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Accepted Answer

NB100-74350 is the same antibody that is conjugated to make NB100-74350B and NB100-74350H. The immunogen for NB100-74350 is the same as NB100-74350B and NB100-74350H.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications	WB, Flow, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-74350AF350

Alexa Fluor 405 NB100-74350AF405

Alexa Fluor 488 NB100-74350AF488

Alexa Fluor 532 NB100-74350AF532

Alexa Fluor 594 NB100-74350AF594

Alexa Fluor 647 NB100-74350AF647

Alexa Fluor 700 NB100-74350AF700

Alexa Fluor 750 NB100-74350AF750

Allophycocyanin NB100-74350APC

Allophycocyanin/Cy7 NB100-74350APCCY7

Biotin NB100-74350B

DyLight 350 NB100-74350UV

DyLight 405 NB100-74350V

DyLight 488 NB100-74350G

DyLight 550 NB100-74350R

DyLight 594 NB100-74350DL594

DyLight 650 NB100-74350C

DyLight 680 NB100-74350FR

DyLight 755 NB100-74350IR

FITC NB100-74350F

HRP NB100-74350H

Janelia Fluor 549 NB100-74350JF549

Janelia Fluor 646 NB100-74350JF646

PE NB100-74350PE

PE/Atto594 NB100-74350PEATT594

PE/Cy5.5 NB100-74350PECY55

PE/Cy7 NB100-74350PECY7

PerCP NB100-74350PCP

mFluor Violet 450 SE NB100-74350MFV450

mFluor Violet 500 SE NB100-74350MFV500

mFluor Violet 610 SE NB100-74350MFV610

Catalog# & Formulation	Size	Price
BSA Free NB100-74350SS

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-74350AF350
Alexa Fluor 405	NB100-74350AF405
Alexa Fluor 488	NB100-74350AF488
Alexa Fluor 532	NB100-74350AF532
Alexa Fluor 594	NB100-74350AF594
Alexa Fluor 647	NB100-74350AF647
Alexa Fluor 700	NB100-74350AF700
Alexa Fluor 750	NB100-74350AF750
Allophycocyanin	NB100-74350APC
Allophycocyanin/Cy7	NB100-74350APCCY7
Biotin	NB100-74350B
DyLight 350	NB100-74350UV
DyLight 405	NB100-74350V
DyLight 488	NB100-74350G
DyLight 550	NB100-74350R
DyLight 594	NB100-74350DL594
DyLight 650	NB100-74350C
DyLight 680	NB100-74350FR
DyLight 755	NB100-74350IR
FITC	NB100-74350F
HRP	NB100-74350H
Janelia Fluor 549	NB100-74350JF549
Janelia Fluor 646	NB100-74350JF646
PE	NB100-74350PE
PE/Atto594	NB100-74350PEATT594
PE/Cy5.5	NB100-74350PECY55
PE/Cy7	NB100-74350PECY7
PerCP	NB100-74350PCP
mFluor Violet 450 SE	NB100-74350MFV450
mFluor Violet 500 SE	NB100-74350MFV500
mFluor Violet 610 SE	NB100-74350MFV610

Aggrecan Neoepitope Antibody - BSA Free

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Publications for Aggrecan Neoepitope Antibody (NB100-74350)(29)

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Immunogen	A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400). [UniProt# P16112]
Specificity	Aggrecan Neo (JSCNIT)
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	ACAN
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	PBS and 30% Glycerol
Preservative	0.1% Sodium Azide
Concentration	1 mg/ml
Purity	Immunogen affinity purified

Publications using NB100-74350	Applications	Species
Song J, Kim EH, Yang JH et al. Upregulated FOXM1 stimulates chondrocyte senescence in Acot12-/-Nudt7-/- double knockout mice Theranostics 2023-09-25 [PMID: 37908734] (IHC-P, Mouse)	IHC-P	Mouse
Dong R, Xu H, Wang P et al. Disruption of Col9a2 expression leads to defects in osteochondral homeostasis and osteoarthritis-like phenotype in mice Journal of Orthopaedic Translation 2023-08-14 [PMID: 37635809] (IHC, ICC/IF)	IHC, ICC/IF
Xiong B, Chen L, Huang Y et al. ZBTB16 eases lipopolysaccharide?elicited inflammation, apoptosis and degradation of extracellular matrix in chondrocytes during osteoarthritis by suppressing GRK2 transcription Experimental and Therapeutic Medicine 2023-04-24 [PMID: 37206562] (WB)	WB
Cui S, Li W, Teixeira G et al. Neoepitope fragments as biomarkers for different phenotypes of intervertebral disc degeneration JOR SPINE 2022-07-06 [PMID: 36203866]
Xu H, Dong R, Zeng Q et al. Col9a2 gene deletion accelerates the degeneration of intervertebral discs Experimental and Therapeutic Medicine 2022-01-07 [PMID: 35126710] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Liu NQ, Lin Y, Li L et al. gp130/STAT3 signaling is required for homeostatic proliferation and anabolism in postnatal growth plate and articular chondrocytes Communications Biology 2022-01-17 [PMID: 35039652] (IHC)	IHC
Oh Hk, Park M, Choi Sw Et Al. Suppression of Osteoarthritis progression by post-natal Induction of Nkx3.2 Biochemical and biophysical research communications 2021-07-27 [PMID: 34330063]
Ding Z, Qiu M, Alharbi M et al FOXO1 expression in chondrocytes modulates cartilage production and removal in fracture healing Bone 2021-03-04 [PMID: 33662610] (FLOW, Mouse) Details: Citation using the Alexa Fluor 488 version of this antibody.	FLOW	Mouse
Ouhaddi Y, Najar M, Paré F, et al. L-PGDS deficiency accelerated the development of naturally occurring age-related osteoarthritis Aging 2020-12-23 [PMID: 33361529] (IHC-P, Mouse)	IHC-P	Mouse
Hozain S, Hernandez A, Fuller J, et al. Zinc chloride affects chondrogenesis via VEGF signaling Experimental cell research 2021-02-15 [PMID: 33358860] (FLOW, Mouse)	FLOW	Mouse
Sugitani K, Egorova D, Mizumoto S et al. Hyaluronan degradation and release of a hyaluronan-aggrecan complex from perineuronal nets in the aged mouse brain Biochim Biophys Acta Gen Subj 2020-11-27 [PMID: 33253804] (WB, Mouse)	WB	Mouse
Bell L, Wallis G, Wilcox K CCN3 (NOV) Drives Degradative Changes in Aging Articular Cartilage Research Square 2020-10-13 [PMID: 33066270] (IF/IHC, Mouse)	IF/IHC	Mouse
Weber, A E, Jalali, O Et al. Modulation of Hedgehog Signaling by Kappa Opioids to Attenuate Osteoarthritis. Arthritis Rheumatol 2020-08-01 [PMID: 32249508] (FLOW, Human) Details: Citation using the Alexa Fluor 405 format of this antibody.	FLOW	Human
Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 2020-04-02 [PMID: 32243809] (Mouse)		Mouse
MitlOhner J, Kaushik R, Niekisch H et al. Dopamine Receptor Activation Modulates the Integrity of the Perisynaptic Extracellular Matrix at Excitatory Synapses Cells 2020-01-21 [PMID: 31972963] (WB, Rat)	WB	Rat
Ho YJ, Lu JW, Ho LJ et al. Anti-inflammatory and anti-osteoarthritis effects of Cm-02 and Ck-02 Biochem. Biophys. Res. Commun. 2019-07-25 [PMID: 31353084]
Xia C, Wang P, Fang L et al. Activation of beta-catenin in Col2-expressing chondrocytes leads to osteoarthritis-like defects in hip joint J. Cell. Physiol. 2019-08-01 [PMID: 30912140] (IF/IHC, Mouse)	IF/IHC	Mouse
Mitlohner J, Kaushik R, Niekisch H, Blondiaux A Dopamine modulates the integrity of the perisynaptic extracellular matrix at excitatory synapses BioRxiv (ICC/IF, Rat)	ICC/IF	Rat
Juneja SC, Ventura M, Jay GD, Veillette C. A Less Invasive Approach of Medial Meniscectomy in Rat: A Model to Target Early or Less Severe Human Osteoarthritis. J Arthritis. 2016-05-27 (IHC-P, Rat)	IHC-P	Rat
Song J, Baek IJ, Chun CH et al. Dysregulation of the NUDT7-PGAM1 axis is responsible for chondrocyte death during osteoarthritis pathogenesis Nat Commun 2018-08-24 [PMID: 30143643] (IF/IHC, Human)	IF/IHC	Human
Shkhyan R, Lee S, Gullo F et al. Genetic ablation of adenosine receptor A3 results in articular cartilage degeneration J. Mol. Med. 2018-08-07 [PMID: 30088034] (WB, Porcine)	WB	Porcine
Liu FC, Lu JW, Chien CY et al. Arthroprotective Effects of Cf-02 Sharing Structural Similarity with Quercetin Int J Mol Sci 2018-05-14 [PMID: 29757957] (IF/IHC, Human)	IF/IHC	Human
Pan Z, Sun H, Xie B et al. Therapeutic effects of gefitinib-encapsulated thermosensitive injectable hydrogel in intervertebral disc degeneration Biomaterials 2018-01-01 [PMID: 29396379] (Mouse)		Mouse
Wang Y, Yu D, Liu Z et al. Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix Stem Cell Res Ther 2017-08-14 [PMID: 28807034] (IHC-P, Mouse)	IHC-P	Mouse
Lai JH, Luo SF, Hung LF et al. Physiological concentrations of soluble uric acid are chondroprotective and anti-inflammatory. Sci Rep 2017-05-24 [PMID: 28539647] (WB, Porcine)	WB	Porcine
Hill Adele, Duran Juanita, Purcell Patricia. Lubricin protects the temporomandibular joint surfaces from degeneration. PLoS One. 2014-09-04 [PMID: 25188282] (IF/IHC)	IF/IHC
Liu FC, Huang HS, Huang CY, Yang R, Chang DM, Lai JH, Ho LJ. A benzamide-linked small molecule HS-Cf inhibits TNF-?-induced interferon regulatory factor-1 in Porcine chondrocytes: a potential disease-modifying drug for osteoarthritis therapeutics. J Clin Immunol;31(6):1131-42. 2011-12-01 [PMID: 21858617] (IF/IHC, Porcine)	IF/IHC	Porcine
Huang CY, Lai KY, Hung LF et al. Advanced glycation end products cause collagen II reduction by activating Janus kinase/signal transducer and activator of transcription 3 pathway in porcine chondrocytes. Rheumatology (Oxford). 2011-08-01 [PMID: 21482542] (FLOW, IHC-Fr, Porcine)	FLOW, IHC-Fr	Porcine
Shaw A Thermogenic regulation of human abdominal and neck area derived adipocytes by mitophagy, irisin and BMP7 Thesis 2022-01-01 (WB, Human) Details: Dilution used for WB 1:1001	WB	Human
Show All 29 Publications. Collapse Publications.

Gene Symbol	ACAN
Entrez	176(Human) 58968(Rat)
Uniprot	P16112()