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TGF-beta 3 Antibody [Biotin]

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Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF-243-NA
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF-243-NA
Carrier Free
AF-243-NA
Carrier Free
AF-243-SP
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TGF-beta 3 Antibody [Biotin] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant chicken TGF‑ beta 3 and recombinant human TGF‑ beta 3
Specificity
Detects TGF-beta 3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) TGF‑ beta 1, rhTGF-beta 1.2, and rhTGF-beta 2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFB3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: TGF-beta 3 Antibody (Catalog # MAB643)
ELISA Detection: TGF-beta 3 Biotinylated Antibody (Catalog # BAF243)
Standard: Recombinant Human TGF-beta 3 (Catalog # 243-B3)
Readout System
Publications
Read Publications using
BAF243 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 3 Antibody [Biotin]

  • ARVD
  • ARVD1
  • FLJ16571
  • LDS5
  • RNHF
  • TGFB3
  • TGFbeta 3
  • TGF-beta 3
  • TGF-beta3
  • TGF-beta-3
  • transforming growth factor beta-3
  • transforming growth factor, beta 3

Background

TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1 - 7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1 - 4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8 - 10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity.(1) TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription ( 3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. Derynck, R. et al. (1988) EMBO J. 7:3737.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGF-beta 3 Antibody (BAF243)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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ELISA Development
(2)
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Human
(2)
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Showing Publications 1 - 6 of 6.
Publications using BAF243 Applications Species
N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype Anticancer Res., 2018-04-01;38(4):2007-2014. 4/1/2018 [PMID: 29599317]
Ryan J. Coram, Samantha J. Stillwagon, Anuradha Guggilam, Michael W. Jenkins, Maurice S. Swanson, Andrea N. Ladd Muscleblind-like 1 is required for normal heart valve development in vivo BMC Developmental Biology 2015-12-01 [PMID: 26472242]
Kameswara Rao Badri, Ming Yue, Oscar A. Carretero, Sree Latha Aramgam, Jun Cao, Stephen Sharkady et al. Blood pressure homeostasis is maintained by a P311–TGF-beta axis Journal of Clinical Investigation 2013-10-01 [PMID: 24091331]
Kathryn E LeMasters, Yotam Blech-Hermoni, Samantha J Stillwagon, Natalie A Vajda, Andrea N Ladd Loss of muscleblind-like 1 promotes invasive mesenchyme formation in endocardial cushions by stimulating autocrine TGF beta 3 BMC Developmental Biology 2012-12-01 [PMID: 22866814]
Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56. 2002-05-01 [PMID: 11943670] (ELISA Development, Human) ELISA Development Human
Simpson H, 2019, Robson SC, Bulmer JN, Barber A, Lyall F Transforming growth factor beta expression in human placenta and placental bed during early pregnancy. 6897, 2002-01-01;23(1):44-58. 2002-01-01 [PMID: 11869091] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol TGFB3