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Tec Antibody (663921)

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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, and BaF3 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Human/Mouse Tec Monoclonal Antibody (Catalog # MAB6519) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tec at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, and BaF3 ...read more
Tec was detected in immersion fixed paraffin-embedded sections of human liver using Human/Mouse Tec Monoclonal Antibody (Catalog # MAB6519) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clone
663921
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6519-SP
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Tec Antibody (663921) Summary

Immunogen
E. coli-derived recombinant human Tec
Met1-Met187
Accession # P42680
Specificity
Detects human Tec in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TEC
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tec Antibody (663921)

  • EC 2.7.10
  • EC 2.7.10.2
  • MGC126760
  • MGC126762
  • PSCTK4
  • PSCTK4tyrosine-protein kinase Tec
  • tec protein tyrosine kinase
  • Tec

Background

The tyrosine protein kinase Tec is an approximately 70 kDa member of the protein kinase superfamily, tyrosine protein family, and Tec subfamily.  Human Tec is 631 amino acids (aa) in length.  Amino acid residues 4-111 constitute a Tec pleckstrin homology (PH) domain, and aa 113-149 make up a Btk-type zinc finger.  Residues 179-239 form a Src homology 3 (SH3) domain, and aa 247-345 make up a Src homology 2 (SH2) domain.  The region involving positions 365-620 constitutes the catalytic domain of the protein tyrosine kinases.  Human Tec shares 94% aa sequence identity with mouse Tec.  Human Tec is expressed in hematopoietic cell lines including B-cell, T-cell, and myeloid lineages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TEC
Uniprot