![Western Blot: SMARCA6 Antibody [NB100-278] - Detection of Human HELLS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and K562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HELLS antibody NB100-278 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMARCA6-Antibody-Western-Blot-NB100-278-img0004.jpg "Western Blot: SMARCA6 Antibody [NB100-278] - Detection of Human HELLS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and K562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HELLS antibody NB100-278 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to a region between residues 550 and 600 of human Helicase, Lymphoid-Specific using the numbering given in entry NP_060533.2 (GeneID 3070). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HELLS |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 97 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publications using NB100-278 | Applications | Species |
|---|---|---|
| Imai Y, Biot M, Cl�ment JA et al. PRDM9 activity depends on HELLS and promotes local 5-hydroxymethylcytosine enrichment eLife 2020-10-13 [PMID: 33047671] | ||
| De Dieuleveult M, Bizet M, Colin L, Calonne E The chromatin remodeler LSH controls genome-wide cytosine hydroxymethylation bioRxiv 2020-01-01 (IP, Human) | IP | Human |
| De Dieuleveult M, Bizet M, Colin L et al. The chromatin remodelling protein LSH/HELLS regulates the amount and distribution of DNA hydroxymethylation in the genome Epigenetics 2021-05-07 [PMID: 33960278] |
Secondary Antibodies |
Isotype Controls |
Research Areas for SMARCA6 Antibody (NB100-278)Find related products by research area.
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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