![Western Blot: BRM Antibody [NBP3-13283] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with BRM antibody (NBP3-13283) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BRM-Antibody-Western-Blot-NBP3-13283-img0007.jpg "Western Blot: BRM Antibody [NBP3-13283] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with BRM antibody (NBP3-13283) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, ChSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human BRM. The exact sequence is proprietary. |
| Predicted Species | Bovine (100%), Chicken (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SMARCA2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SMARCA2 |
![Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleolus by immunofluorescent analysis. Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRM stained by BRM antibody (NBP3-13283) diluted at 1:500. Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13283-img0004.jpg "Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleolus by immunofluorescent analysis. Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRM stained by BRM antibody (NBP3-13283) diluted at 1:500. Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.")
![Immunohistochemistry-Paraffin: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleus in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. BRM antibody (NBP3-13283) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/BRM-Antibody-Immunohistochemistry-Paraffin-NBP3-13283-img0002.jpg "Immunohistochemistry-Paraffin: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleus in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. BRM antibody (NBP3-13283) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: BRM Antibody [NBP3-13283] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRM antibody (NBP3-13283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/BRM-Antibody-Western-Blot-NBP3-13283-img0005.jpg "Western Blot: BRM Antibody [NBP3-13283] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRM antibody (NBP3-13283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Western Blot: BRM Antibody [NBP3-13283] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with BRM antibody (NBP3-13283) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BRM-Antibody-Western-Blot-NBP3-13283-img0006.jpg "Western Blot: BRM Antibody [NBP3-13283] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with BRM antibody (NBP3-13283) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRM stained by BRM antibody (NBP3-13283) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](http://images.novusbio.com/fullsize/BRM-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13283-img0003.jpg "Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRM stained by BRM antibody (NBP3-13283) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BRM antibody (NBP3-13283) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/BRM-Antibody-Immunohistochemistry-Paraffin-NBP3-13283-img0001.jpg "Immunohistochemistry-Paraffin: BRM Antibody [NBP3-13283] - BRM antibody detects BRM protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BRM antibody (NBP3-13283) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")